7BXG

MavC-UBE2N-Ub complex

7BXG の概要

• エントリーDOI: 10.2210/pdb7bxg/pdb
• 分子名称: MavC, Ubiquitin-conjugating enzyme E2 N, Polyubiquitin-C, ... (4 entities in total)
• 機能のキーワード: ubiquitination, deubiquitination, mavc, mvca, lpg2149, ube2n, transferase
• 由来する生物種: Legionella pneumophila subsp. pneumophila str. Philadelphia 1; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 69908.51

構造登録者

Gao, P.,Wang, Y. (登録日: 2020-04-19, 公開日: 2020-05-20, 最終更新日: 2023-11-29)

主引用文献

Wang, Y.,Zhan, Q.,Wang, X.,Li, P.,Liu, S.,Gao, G.,Gao, P.
Insights into catalysis and regulation of non-canonical ubiquitination and deubiquitination by bacterial deamidase effectors.
Nat Commun, 11:2751-2751, 2020

PubMed Abstract: The bacterial effector MavC catalyzes non-canonical ubiquitination of host E2 enzyme UBE2N without engaging any of the conventional ubiquitination machinery, thereby abolishing UBE2N's function in forming K63-linked ubiquitin (Ub) chains and dampening NF-кB signaling. We now report the structures of MavC in complex with conjugated UBE2N~Ub and an inhibitor protein Lpg2149, as well as the structure of its ortholog, MvcA, bound to Lpg2149. Recognition of UBE2N and Ub depends on several unique features of MavC, which explains the inability of MvcA to catalyze ubiquitination. Unexpectedly, MavC and MvcA also possess deubiquitinase activity against MavC-mediated ubiquitination, highlighting MavC as a unique enzyme possessing deamidation, ubiquitination, and deubiquitination activities. Further, Lpg2149 directly binds and inhibits both MavC and MvcA by disrupting the interactions between enzymes and Ub. These results provide detailed insights into catalysis and regulation of MavC-type enzymes and the molecular mechanisms of this non-canonical ubiquitination machinery.

PubMed: 32488130
DOI: 10.1038/s41467-020-16587-w

実験手法

X-RAY DIFFRACTION (2.705 Å)