7BX7

Cryo-EM structure of amyloid fibril formed by hnRNPA1 low complexity domain

7BX7 の概要

• エントリーDOI: 10.2210/pdb7bx7/pdb
• EMDBエントリー: 30235
• 分子名称: Heterogeneous nuclear ribonucleoprotein A1 (1 entity in total)
• 機能のキーワード: amyloid fibril, protein fibril
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 78494.53

構造登録者

Sun, Y.P.,Zhao, K.,Liu, C.,Li, D. (登録日: 2020-04-17, 公開日: 2020-11-18, 最終更新日: 2024-03-27)

主引用文献

Sun, Y.,Zhao, K.,Xia, W.,Feng, G.,Gu, J.,Ma, Y.,Gui, X.,Zhang, X.,Fang, Y.,Sun, B.,Wang, R.,Liu, C.,Li, D.
The nuclear localization sequence mediates hnRNPA1 amyloid fibril formation revealed by cryoEM structure.
Nat Commun, 11:6349-6349, 2020

PubMed Abstract: Human heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) serves as a key regulating protein in RNA metabolism. Malfunction of hnRNPA1 in nucleo-cytoplasmic transport or dynamic phase separation leads to abnormal amyloid aggregation and neurodegeneration. The low complexity (LC) domain of hnRNPA1 drives both dynamic phase separation and amyloid aggregation. Here, we use cryo-electron microscopy to determine the amyloid fibril structure formed by hnRNPA1 LC domain. Remarkably, the structure reveals that the nuclear localization sequence of hnRNPA1 (termed PY-NLS), which is initially known to mediate the nucleo-cytoplamic transport of hnRNPA1 through binding with karyopherin-β2 (Kapβ2), represents the major component of the fibril core. The residues that contribute to the binding of PY-NLS with Kapβ2 also exert key molecular interactions to stabilize the fibril structure. Notably, hnRNPA1 mutations found in familial amyotrophic lateral sclerosis (ALS) and multisystem proteinopathoy (MSP) are all involved in the fibril core and contribute to fibril stability. Our work illuminates structural understandings of the pathological amyloid aggregation of hnRNPA1 and the amyloid disaggregase activity of Kapβ2, and highlights the multiple roles of PY-NLS in hnRNPA1 homeostasis.

PubMed: 33311513
DOI: 10.1038/s41467-020-20227-8

実験手法

ELECTRON MICROSCOPY (2.8 Å)