7BWK

Structure of DotL(656-783)-IcmS-IcmW-LvgA-VpdB(461-590) derived from Legionella pneumophila

7BWK の概要

• エントリーDOI: 10.2210/pdb7bwk/pdb
• 分子名称: IcmO (DotL), IcmS, IcmW, ... (5 entities in total)
• 機能のキーワード: dot/icm type 4b secretion system, effector recognition, type 4b coupling protein complex-effector complex, dotl-icmsw-lvga, vpdb, protein transport
• 由来する生物種: Legionella pneumophila subsp. pneumophila str. Philadelphia 1; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 165249.83

構造登録者

Kim, H.,Kwak, M.J.,Oh, B.H. (登録日: 2020-04-14, 公開日: 2020-06-03, 最終更新日: 2023-11-29)

主引用文献

Kim, H.,Kubori, T.,Yamazaki, K.,Kwak, M.J.,Park, S.Y.,Nagai, H.,Vogel, J.P.,Oh, B.H.
Structural basis for effector protein recognition by the Dot/Icm Type IVB coupling protein complex.
Nat Commun, 11:2623-2623, 2020

PubMed Abstract: The Legionella pneumophila Dot/Icm type IVB secretion system (T4BSS) is extremely versatile, translocating ~300 effector proteins into host cells. This specialized secretion system employs the Dot/Icm type IVB coupling protein (T4CP) complex, which includes IcmS, IcmW and LvgA, that are known to selectively assist the export of a subclass of effectors. Herein, the crystal structure of a four-subunit T4CP subcomplex bound to the effector protein VpdB reveals an interaction between LvgA and a linear motif in the C-terminus of VpdB. The same binding interface of LvgA also interacts with the C-terminal region of three additional effectors, SidH, SetA and PieA. Mutational analyses identified a FxxxLxxxK binding motif that is shared by VpdB and SidH, but not by SetA and PieA, showing that LvgA recognizes more than one type of binding motif. Together, this work provides a structural basis for how the Dot/Icm T4CP complex recognizes effectors, and highlights the multiple substrate-binding specificities of its adaptor subunit.

PubMed: 32457311
DOI: 10.1038/s41467-020-16397-0

実験手法

X-RAY DIFFRACTION (2.801 Å)