7BV1

Cryo-EM structure of the apo nsp12-nsp7-nsp8 complex

7BV1 の概要

• エントリーDOI: 10.2210/pdb7bv1/pdb
• EMDBエントリー: 30209
• 分子名称: RNA-directed RNA polymerase, Non-structural protein 8, Non-structural protein 7, ... (4 entities in total)
• 機能のキーワード: sars-cov-2, rna polymerase, apo, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 165965.28

構造登録者

Yin, W.,Mao, C.,Luan, X.,Shen, D.,Shen, Q.,Su, H.,Wang, X.,Zhou, F.,Zhao, W.,Gao, M.,Chang, S.,Xie, Y.C.,Tian, G.,Jiang, H.W.,Tao, S.C.,Shen, J.,Jiang, Y.,Jiang, H.,Xu, Y.,Zhang, S.,Zhang, Y.,Xu, H.E. (登録日: 2020-04-09, 公開日: 2020-04-22, 最終更新日: 2024-03-27)

主引用文献

Yin, W.,Mao, C.,Luan, X.,Shen, D.D.,Shen, Q.,Su, H.,Wang, X.,Zhou, F.,Zhao, W.,Gao, M.,Chang, S.,Xie, Y.C.,Tian, G.,Jiang, H.W.,Tao, S.C.,Shen, J.,Jiang, Y.,Jiang, H.,Xu, Y.,Zhang, S.,Zhang, Y.,Xu, H.E.
Structural basis for inhibition of the RNA-dependent RNA polymerase from SARS-CoV-2 by remdesivir.
Science, 368:1499-1504, 2020

PubMed Abstract: The pandemic of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a global crisis. Replication of SARS-CoV-2 requires the viral RNA-dependent RNA polymerase (RdRp) enzyme, a target of the antiviral drug remdesivir. Here we report the cryo-electron microscopy structure of the SARS-CoV-2 RdRp, both in the apo form at 2.8-angstrom resolution and in complex with a 50-base template-primer RNA and remdesivir at 2.5-angstrom resolution. The complex structure reveals that the partial double-stranded RNA template is inserted into the central channel of the RdRp, where remdesivir is covalently incorporated into the primer strand at the first replicated base pair, and terminates chain elongation. Our structures provide insights into the mechanism of viral RNA replication and a rational template for drug design to combat the viral infection.

PubMed: 32358203
DOI: 10.1126/science.abc1560

実験手法

ELECTRON MICROSCOPY (2.8 Å)