7BRT

Crystal structure of Sec62 LIR fused to GABARAP

Summary for 7BRT

• Entry DOI: 10.2210/pdb7brt/pdb
• Related: 7BRN 7BRQ
• Descriptor: Translocation protein SEC62,Gamma-aminobutyric acid receptor-associated protein (2 entities in total)
• Functional Keywords: autophagy, endoplasmic reticulum, membrane protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 31783.97

Authors

Yamasaki, A.,Noda, N.N. (deposition date: 2020-03-30, release date: 2020-07-08, Last modification date: 2023-11-29)

Primary citation

Mochida, K.,Yamasaki, A.,Matoba, K.,Kirisako, H.,Noda, N.N.,Nakatogawa, H.
Super-assembly of ER-phagy receptor Atg40 induces local ER remodeling at contacts with forming autophagosomal membranes.
Nat Commun, 11:3306-3306, 2020

PubMed Abstract: The endoplasmic reticulum (ER) is selectively degraded by autophagy (ER-phagy) through proteins called ER-phagy receptors. In Saccharomyces cerevisiae, Atg40 acts as an ER-phagy receptor to sequester ER fragments into autophagosomes by binding Atg8 on forming autophagosomal membranes. During ER-phagy, parts of the ER are morphologically rearranged, fragmented, and loaded into autophagosomes, but the mechanism remains poorly understood. Here we find that Atg40 molecules assemble in the ER membrane concurrently with autophagosome formation via multivalent interaction with Atg8. Atg8-mediated super-assembly of Atg40 generates highly-curved ER regions, depending on its reticulon-like domain, and supports packing of these regions into autophagosomes. Moreover, tight binding of Atg40 to Atg8 is achieved by a short helix C-terminal to the Atg8-family interacting motif, and this feature is also observed for mammalian ER-phagy receptors. Thus, this study significantly advances our understanding of the mechanisms of ER-phagy and also provides insights into organelle fragmentation in selective autophagy of other organelles.

PubMed: 32620754
DOI: 10.1038/s41467-020-17163-y

Experimental method

X-RAY DIFFRACTION (1.999 Å)