7BNY

Structure of 2A protein from encephalomyocarditis virus (EMCV)

7BNY の概要

• エントリーDOI: 10.2210/pdb7bny/pdb
• 分子名称: Genome polyprotein, SULFATE ION (3 entities in total)
• 機能のキーワード: emcv, cardiovirus, 2a, picornavirus, frameshifting, prf, rna-binding protein, protein-mediated frameshifting, ribosome-binding protein, beta-shell, viral protein
• 由来する生物種: Mengo encephalomyocarditis virus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 72189.29

構造登録者

Hill, C.H.,Napthine, S.,Pekarek, L.,Kibe, A.,Firth, A.E.,Graham, S.C.,Caliskan, N.,Brierley, I. (登録日: 2021-01-22, 公開日: 2021-12-08, 最終更新日: 2022-02-02)

主引用文献

Hill, C.H.,Pekarek, L.,Napthine, S.,Kibe, A.,Firth, A.E.,Graham, S.C.,Caliskan, N.,Brierley, I.
Structural and molecular basis for Cardiovirus 2A protein as a viral gene expression switch.
Nat Commun, 12:7166-7166, 2021

PubMed Abstract: Programmed -1 ribosomal frameshifting (PRF) in cardioviruses is activated by the 2A protein, a multi-functional virulence factor that also inhibits cap-dependent translational initiation. Here we present the X-ray crystal structure of 2A and show that it selectively binds to a pseudoknot-like conformation of the PRF stimulatory RNA element in the viral genome. Using optical tweezers, we demonstrate that 2A stabilises this RNA element, likely explaining the increase in PRF efficiency in the presence of 2A. Next, we demonstrate a strong interaction between 2A and the small ribosomal subunit and present a cryo-EM structure of 2A bound to initiated 70S ribosomes. Multiple copies of 2A bind to the 16S rRNA where they may compete for binding with initiation and elongation factors. Together, these results define the structural basis for RNA recognition by 2A, show how 2A-mediated stabilisation of an RNA pseudoknot promotes PRF, and reveal how 2A accumulation may shut down translation during virus infection.

PubMed: 34887415
DOI: 10.1038/s41467-021-27400-7

実験手法

X-RAY DIFFRACTION (2.62 Å)