7BNW

Llama nanobody nb18 raised against GldL from Flavobacterium jonhsoniae

7BNW の概要

• エントリーDOI: 10.2210/pdb7bnw/pdb
• 分子名称: nanobody nb18 (2 entities in total)
• 機能のキーワード: nanobody, type ix secretion system, immune system
• 由来する生物種: Lama glama (Llama)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 57344.16

構造登録者

Trinh, N.T.,Cambillau, C.,Leone, P. (登録日: 2021-01-22, 公開日: 2021-05-26, 最終更新日: 2024-11-13)

主引用文献

Trinh, T.T.N.,Gaubert, A.,Melani, P.,Cambillau, C.,Roussel, A.,Leone, P.
Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae.
Acta Crystallogr.,Sect.F, 77:171-176, 2021

PubMed Abstract: GldL is an inner-membrane protein that is essential for the function of the type IX secretion system (T9SS) in Flavobacterium johnsoniae. The complex that it forms with GldM is supposed to act as a new rotary motor involved in the gliding motility of the bacterium. In the context of structural studies of GldL to gain information on the assembly and function of the T9SS, two camelid nanobodies were selected, produced and purified. Their interaction with the cytoplasmic domain of GldL was characterized and their crystal structures were solved. These nanobodies will be used as crystallization chaperones to help in the crystallization of the cytoplasmic domain of GldL and could also help to solve the structure of the complex using molecular replacement.

PubMed: 34100775
DOI: 10.1107/S2053230X21005185

実験手法

X-RAY DIFFRACTION (2.59 Å)