7BME
Crystal structure of a R18W mutant of the DNA-binding protein RemA from Geobacillus thermodenitrificans
Summary for 7BME
| Entry DOI | 10.2210/pdb7bme/pdb |
| Related | 7BM2 |
| Descriptor | Putative regulatory protein GTNG_1019 (2 entities in total) |
| Functional Keywords | biofilm, dna-binding, histone-like, geobacillus, dna binding protein |
| Biological source | Geobacillus thermodenitrificans (strain NG80-2) |
| Total number of polymer chains | 7 |
| Total formula weight | 73527.37 |
| Authors | Altegoer, F.,Mrusek, D.,Bange, G. (deposition date: 2021-01-20, release date: 2021-08-25, Last modification date: 2024-01-31) |
| Primary citation | Hoffmann, T.,Mrusek, D.,Bedrunka, P.,Burchert, F.,Mais, C.N.,Kearns, D.B.,Altegoer, F.,Bremer, E.,Bange, G. Structural and functional characterization of the bacterial biofilm activator RemA. Nat Commun, 12:5707-5707, 2021 Cited by PubMed Abstract: Bacillus subtilis can form structurally complex biofilms on solid or liquid surfaces, which requires expression of genes for matrix production. The transcription of these genes is activated by regulatory protein RemA, which binds to poorly conserved, repetitive DNA regions but lacks obvious DNA-binding motifs or domains. Here, we present the structure of the RemA homologue from Geobacillus thermodenitrificans, showing a unique octameric ring with the potential to form a 16-meric superstructure. These results, together with further biochemical and in vivo characterization of B. subtilis RemA, suggests that the protein can wrap DNA around its ring-like structure through a LytTR-related domain. PubMed: 34588455DOI: 10.1038/s41467-021-26005-4 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.6 Å) |
Structure validation
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