7BKC

Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from Methanospirillum hungatei (dimeric, composite structure)

7BKC の概要

• エントリーDOI: 10.2210/pdb7bkc/pdb
• 関連するPDBエントリー: 7BKB 7BKD 7BKE
• EMDBエントリー: 12196 12197 12198 12199 12206 12209 12210 12211 12212
• 分子名称: CoB--CoM heterodisulfide reductase iron-sulfur subunit A, Formylmethanofuran dehydrogenase, subunit D, Formylmethanofuran dehydrogenase, subunit F, ... (19 entities in total)
• 機能のキーワード: methanogenesis, flavin-based electron bifurcation, co2-fixation, formate dehydrogenase, oxidoreductase
• 由来する生物種: Methanospirillum hungatei JF-1; 詳細
• タンパク質・核酸の鎖数: 26
• 化学式量合計: 1070095.69

構造登録者

Pfeil-Gardiner, O.,Watanabe, T.,Shima, S.,Murphy, B.J. (登録日: 2021-01-15, 公開日: 2021-09-29)

主引用文献

Watanabe, T.,Pfeil-Gardiner, O.,Kahnt, J.,Koch, J.,Shima, S.,Murphy, B.J.
Three-megadalton complex of methanogenic electron-bifurcating and CO 2 -fixing enzymes.
Science, 373:1151-1156, 2021

PubMed Abstract: The first reaction of the methanogenic pathway from carbon dioxide (CO) is the reduction and condensation of CO to formyl-methanofuran, catalyzed by formyl-methanofuran dehydrogenase (Fmd). Strongly reducing electrons for this reaction are generated by heterodisulfide reductase (Hdr) in complex with hydrogenase or formate dehydrogenase (Fdh) using a flavin-based electron-bifurcation mechanism. Here, we report enzymological and structural characterizations of Fdh-Hdr-Fmd complexes from . The complexes catalyze this reaction using electrons from formate and the reduced form of the electron carrier F. Conformational changes in HdrA mediate electron bifurcation, and polyferredoxin FmdF directly transfers electrons to the CO reduction site, as evidenced by methanofuran-dependent flavin-based electron bifurcation even without free ferredoxin, a diffusible electron carrier between Hdr and Fmd. Conservation of Hdr and Fmd structures suggests that this complex is common among hydrogenotrophic methanogens.

PubMed: 34516836
DOI: 10.1126/science.abg5550

実験手法

ELECTRON MICROSCOPY (3 Å)