7BIO

Crystal structure of monooxygenase RslO4 from Streptomyces bottropensis

7BIO の概要

• エントリーDOI: 10.2210/pdb7bio/pdb
• 分子名称: Monooxygenase/putative anthronoxygenase, ETHANOL, PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: rishirilides biosynthesis, oxidoreductase
• 由来する生物種: Streptomyces bottropensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26771.90

構造登録者

Zhang, L.,Zuo, C.,Alali, A.,Bechthold, A.,Einsle, O. (登録日: 2021-01-12, 公開日: 2021-01-20, 最終更新日: 2024-01-31)

主引用文献

Alali, A.,Zhang, L.,Li, J.,Zuo, C.,Wassouf, D.,Yan, X.,Schwarzer, P.,Gunther, S.,Einsle, O.,Bechthold, A.
Biosynthesis of the Tricyclic Aromatic Type II Polyketide Rishirilide: New Potential Third Ring Oxygenation after Three Cyclization Steps.
Mol Biotechnol., 63:502-514, 2021

PubMed Abstract: Rishirilides are a group of PKS II secondary metabolites produced by Streptomyces bottropensis Gö C4/4. Biosynthetic studies in the past have elucidated early and late steps of rishirilide biosynthesis. This work is aiming to solve the remaining steps in the rishirilide biosynthesis. Inactivation of the cyclase gene rslC3 in Streptomyces bottropensis resulted in an interruption of rishirilide production. Instead, accumulation of the tricyclic aromatic galvaquinones was observed. Similar results were observed after deletion of rslO4. Closer inspection into RslO4 crystal structure in addition to site-directed mutagenesis and molecular dynamic simulations revealed that RslO4 might be responsible for quinone formation on the third ring. The RslO1 three-dimensional structure shows a high similarity to FMN-dependent luciferase-like monooxygenases such as the epoxy-forming MsnO8 which acts with the flavin reductase MsnO3 in mensacarcin biosynthesis in the same strain. The high sequence similarity between RslO2 and MsnO3 suggests that RslO2 provides RslO1 with reduced FMN to form an epoxide that serves as substrate for RslO5.

PubMed: 33763824
DOI: 10.1007/s12033-021-00314-x

実験手法

X-RAY DIFFRACTION (1.795 Å)