7BHT

Crystal structure of MAT2a with quinazolinone fragment 5 bound in the allosteric site

7BHT の概要

• エントリーDOI: 10.2210/pdb7bht/pdb
• 分子名称: S-adenosylmethionine synthase isoform type-2, DIMETHYL SULFOXIDE, 7-chloranyl-4-(dimethylamino)-1~{H}-quinazolin-2-one, ... (6 entities in total)
• 機能のキーワード: allosteric inhibitor, fragment-based drug design, synthetic lethal therapy, oncology, transferase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44671.51

構造登録者

Schimpl, M.,De Fusco, C.,Borjesson, U.,Cheung, T.,Collie, I.,Evans, L.,Narasimhan, P.,Stubbs, C.,Vazquez-Chantada, M.,Wagner, D.J.,Grondine, M.,Tentarelli, S.,Underwood, E.,Argyrou, A.,Bagal, S.,Chiarparin, E.,Robb, G.,Scott, J.S. (登録日: 2021-01-11, 公開日: 2021-04-21, 最終更新日: 2024-05-01)

主引用文献

De Fusco, C.,Schimpl, M.,Borjesson, U.,Cheung, T.,Collie, I.,Evans, L.,Narasimhan, P.,Stubbs, C.,Vazquez-Chantada, M.,Wagner, D.J.,Grondine, M.,Sanders, M.G.,Tentarelli, S.,Underwood, E.,Argyrou, A.,Smith, J.M.,Lynch, J.T.,Chiarparin, E.,Robb, G.,Bagal, S.K.,Scott, J.S.
Fragment-Based Design of a Potent MAT2a Inhibitor and in Vivo Evaluation in an MTAP Null Xenograft Model.
J.Med.Chem., 64:6814-6826, 2021

PubMed Abstract: MAT2a is a methionine adenosyltransferase that synthesizes the essential metabolite -adenosylmethionine (SAM) from methionine and ATP. Tumors bearing the co-deletion of p16 and MTAP genes have been shown to be sensitive to MAT2a inhibition, making it an attractive target for treatment of MTAP-deleted cancers. A fragment-based lead generation campaign identified weak but efficient hits binding in a known allosteric site. By use of structure-guided design and systematic SAR exploration, the hits were elaborated through a merging and growing strategy into an arylquinazolinone series of potent MAT2a inhibitors. The selected tool compound reduced SAM-dependent methylation events in cells and inhibited proliferation of MTAP-null cells . studies showed that was able to induce antitumor response in an MTAP knockout HCT116 xenograft model.

PubMed: 33900758
DOI: 10.1021/acs.jmedchem.1c00067

実験手法

X-RAY DIFFRACTION (1.052 Å)