7BHO

DNA origami signpost designed model

これはPDB形式変換不可エントリーです。

7BHO の概要

• エントリーDOI: 10.2210/pdb7bho/pdb
• EMDBエントリー: 12188
• 分子名称: DNA, DNA scaffold, ... (238 entities in total)
• 機能のキーワード: origami, nanostructure, signpost, spot, dna
• 由来する生物種: Escherichia virus M13; 詳細
• タンパク質・核酸の鎖数: 238
• 化学式量合計: 4453172.71

構造登録者

Silvester, E.,Vollmer, B.,Prazak, V.,Vasishtan, D.,Machala, E.A.,Whittle, C.,Black, S.,Bath, J.,Turberfield, A.J.,Gruenewald, K.,Baker, L.A. (登録日: 2021-01-11, 公開日: 2021-04-14, 最終更新日: 2024-05-01)

主引用文献

Silvester, E.,Vollmer, B.,Prazak, V.,Vasishtan, D.,Machala, E.A.,Whittle, C.,Black, S.,Bath, J.,Turberfield, A.J.,Grunewald, K.,Baker, L.A.
DNA origami signposts for identifying proteins on cell membranes by electron cryotomography.
Cell, 184:1110-1121.e16, 2021

PubMed Abstract: Electron cryotomography (cryoET), an electron cryomicroscopy (cryoEM) modality, has changed our understanding of biological function by revealing the native molecular details of membranes, viruses, and cells. However, identification of individual molecules within tomograms from cryoET is challenging because of sample crowding and low signal-to-noise ratios. Here, we present a tagging strategy for cryoET that precisely identifies individual protein complexes in tomograms without relying on metal clusters. Our method makes use of DNA origami to produce "molecular signposts" that target molecules of interest, here via fluorescent fusion proteins, providing a platform generally applicable to biological surfaces. We demonstrate the specificity of signpost origami tags (SPOTs) in vitro as well as their suitability for cryoET of membrane vesicles, enveloped viruses, and the exterior of intact mammalian cells.

PubMed: 33606980
DOI: 10.1016/j.cell.2021.01.033

実験手法

ELECTRON MICROSCOPY (36.44 Å)