7BD7

X-ray structure of Arsenoplatin-1-encapsulated horse spleen ferritin

7BD7 の概要

• エントリーDOI: 10.2210/pdb7bd7/pdb
• 分子名称: Ferritin light chain, SODIUM ION, CADMIUM ION, ... (8 entities in total)
• 機能のキーワード: ferritin, drug delivery, metallodrug, arsenoplatin, anticancer, transport protein
• 由来する生物種: Equus caballus (Horse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22108.15

構造登録者

Ferraro, G.,Merlino, A. (登録日: 2020-12-21, 公開日: 2021-03-17, 最終更新日: 2024-01-31)

主引用文献

Ferraro, G.,Pratesi, A.,Cirri, D.,Imbimbo, P.,Maria Monti, D.,Messori, L.,Merlino, A.
Arsenoplatin-Ferritin Nanocage: Structure and Cytotoxicity.
Int J Mol Sci, 22:-, 2021

PubMed Abstract: Arsenoplatin-1 (AP-1), the prototype of a novel class of metallodrugs containing a PtAs(OH) core, was encapsulated within the apoferritin (AFt) nanocage. UV-Vis absorption spectroscopy and inductively coupled plasma-atomic emission spectroscopy measurements confirmed metallodrug encapsulation and allowed us to determine the average amount of AP-1 trapped inside the cage. The X-ray structure of AP-1-encapsulated AFt was solved at 1.50 Å. Diffraction data revealed that an AP-1 fragment coordinates the side chain of a His residue. The biological activity of AP-1-loaded AFt was comparatively tested on a few representative cancer and non-cancer cell lines. Even though the presence of the cage reduces the overall cytotoxicity of AP-1, it improves its selectivity towards cancer cells.

PubMed: 33668605
DOI: 10.3390/ijms22041874

実験手法

X-RAY DIFFRACTION (1.5 Å)