7BBL

Structure of human Gemin6/Gemin7/Gemin8 trimeric complex

7BBL の概要

• エントリーDOI: 10.2210/pdb7bbl/pdb
• 分子名称: Gem-associated protein 6, Gem-associated protein 7, Gem-associated protein 8, ... (4 entities in total)
• 機能のキーワード: usnrnp, biogenesis, smn-complex, gemin8, splicing
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 50343.37

構造登録者

Viswanathan, A.,Grimm, C.,Fischer, U. (登録日: 2020-12-17, 公開日: 2021-01-20, 最終更新日: 2024-01-31)

主引用文献

Veepaschit, J.,Viswanathan, A.,Bordonne, R.,Grimm, C.,Fischer, U.
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex.
Nucleic Acids Res., 49:7207-7223, 2021

PubMed Abstract: The macromolecular SMN complex facilitates the formation of Sm-class ribonucleoproteins involved in mRNA processing (UsnRNPs). While biochemical studies have revealed key activities of the SMN complex, its structural investigation is lagging behind. Here we report on the identification and structural determination of the SMN complex from the lower eukaryote Schizosaccharomyces pombe, consisting of SMN, Gemin2, 6, 7, 8 and Sm proteins. The core of the SMN complex is formed by several copies of SMN tethered through its C-terminal alpha-helices arranged with alternating polarity. This creates a central platform onto which Gemin8 binds and recruits Gemins 6 and 7. The N-terminal parts of the SMN molecules extrude via flexible linkers from the core and enable binding of Gemin2 and Sm proteins. Our data identify the SMN complex as a multivalent hub where Sm proteins are collected in its periphery to allow their joining with UsnRNA.

PubMed: 33754639
DOI: 10.1093/nar/gkab158

実験手法

X-RAY DIFFRACTION (1.521 Å)