7BB7

AVP-V2R-Galphas-beta1-gamma2-Nb35(T state)

7BB7 の概要

• エントリーDOI: 10.2210/pdb7bb7/pdb
• EMDBエントリー: 12129
• 分子名称: Vasopressin V2 receptor, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, ... (6 entities in total)
• 機能のキーワード: signaling protein, g protein-coupled receptor, hormrne, vasopressin, v2r, membrane protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 158206.95

構造登録者

Bous, J.,Mouillac, B.,Bron, P.,Granier, S.,Floquet, N.,Leyrat, C. (登録日: 2020-12-17, 公開日: 2021-06-02, 最終更新日: 2024-11-13)

主引用文献

Bous, J.,Orcel, H.,Floquet, N.,Leyrat, C.,Lai-Kee-Him, J.,Gaibelet, G.,Ancelin, A.,Saint-Paul, J.,Trapani, S.,Louet, M.,Sounier, R.,Demene, H.,Granier, S.,Bron, P.,Mouillac, B.
Cryo-electron microscopy structure of the antidiuretic hormone arginine-vasopressin V2 receptor signaling complex.
Sci Adv, 7:-, 2021

PubMed Abstract: The antidiuretic hormone arginine-vasopressin (AVP) forms a signaling complex with the V2 receptor (V2R) and the G protein, promoting kidney water reabsorption. Molecular mechanisms underlying activation of this critical G protein-coupled receptor (GPCR) signaling system are still unknown. To fill this gap of knowledge, we report here the cryo-electron microscopy structure of the AVP-V2R-G complex. Single-particle analysis revealed the presence of three different states. The two best maps were combined with computational and nuclear magnetic resonance spectroscopy constraints to reconstruct two structures of the ternary complex. These structures differ in AVP and G binding modes. They reveal an original receptor-G interface in which the Gα subunit penetrates deep into the active V2R. The structures help to explain how V2R R137H or R137L/C variants can lead to two severe genetic diseases. Our study provides important structural insights into the function of this clinically relevant GPCR signaling complex.

PubMed: 34020960
DOI: 10.1126/sciadv.abg5628

実験手法

ELECTRON MICROSCOPY (4.4 Å)