7B7U

Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2

7B7U の概要

• エントリーDOI: 10.2210/pdb7b7u/pdb
• EMDBエントリー: 12087
• 分子名称: DNA-directed RNA polymerase subunit, RNA polymerase II subunit K, Putative RNA polymerase II subunit B1 CTD phosphatase RPAP2, ... (13 entities in total)
• 機能のキーワード: rpap2, rna polymerase ii, nuclear import, transcription
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 553875.91

構造登録者

Fianu, I.,Dienemann, C.,Aibara, S.,Schilbach, S.,Cramer, P. (登録日: 2020-12-11, 公開日: 2021-05-19, 最終更新日: 2025-12-17)

主引用文献

Fianu, I.,Dienemann, C.,Aibara, S.,Schilbach, S.,Cramer, P.
Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2.
Commun Biol, 4:606-606, 2021

PubMed Abstract: Nuclear import of RNA polymerase II (Pol II) involves the conserved factor RPAP2. Here we report the cryo-electron microscopy (cryo-EM) structure of mammalian Pol II in complex with human RPAP2 at 2.8 Å resolution. The structure shows that RPAP2 binds between the jaw domains of the polymerase subunits RPB1 and RPB5. RPAP2 is incompatible with binding of downstream DNA during transcription and is displaced upon formation of a transcription pre-initiation complex.

PubMed: 34021257
DOI: 10.1038/s42003-021-02088-z

実験手法

ELECTRON MICROSCOPY (2.8 Å)