7B5H

Cryo-EM structure of the contractile injection system base plate from Anabaena PCC7120

これはPDB形式変換不可エントリーです。

7B5H の概要

• エントリーDOI: 10.2210/pdb7b5h/pdb
• 関連するPDBエントリー: 7B5I
• EMDBエントリー: 12029 12030 12031 12032 12033 12034
• 分子名称: All3314 protein, All3324 protein, All3325 protein, ... (11 entities in total)
• 機能のキーワード: contractile tail, injection system, macromolecular machine, protein transport
• 由来する生物種: Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576); 詳細
• タンパク質・核酸の鎖数: 96
• 化学式量合計: 6722331.56

構造登録者

Eisenstein, F.,Weiss, G.L.,Pilhofer, M. (登録日: 2020-12-03, 公開日: 2022-02-23, 最終更新日: 2024-11-13)

主引用文献

Weiss, G.L.,Eisenstein, F.,Kieninger, A.K.,Xu, J.,Minas, H.A.,Gerber, M.,Feldmuller, M.,Maldener, I.,Forchhammer, K.,Pilhofer, M.
Structure of a thylakoid-anchored contractile injection system in multicellular cyanobacteria.
Nat Microbiol, 7:386-396, 2022

PubMed Abstract: Contractile injection systems (CISs) mediate cell-cell interactions by phage tail-like structures, using two distinct modes of action: extracellular CISs are released into the medium, while type 6 secretion systems (T6SSs) are attached to the cytoplasmic membrane and function upon cell-cell contact. Here, we characterized a CIS in the multicellular cyanobacterium Anabaena, with features distinct from extracellular CISs and T6SSs. Cryo-electron tomography of focused ion beam-milled cells revealed that CISs were anchored in thylakoid membrane stacks, facing the cell periphery. Single particle cryo-electron microscopy showed that this unique in situ localization was mediated by extensions of tail fibre and baseplate components. On stress, cyanobacteria induced the formation of ghost cells, presenting thylakoid-anchored CISs to the environment. Functional assays suggest that these CISs may mediate ghost cell formation and/or interactions of ghost cells with other organisms. Collectively, these data provide a framework for understanding the evolutionary re-engineering of CISs and potential roles of these CISs in cyanobacterial programmed cell death.

PubMed: 35165386
DOI: 10.1038/s41564-021-01055-y

実験手法

ELECTRON MICROSCOPY (3.2 Å)