7B4J

Thermostable omega transaminase PjTA-R6 variant W58M/F86L/R417L engineered for asymmetric synthesis of enantiopure bulky amines

7B4J の概要

• エントリーDOI: 10.2210/pdb7b4j/pdb
• 分子名称: Aspartate aminotransferase family protein, 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE, SUCCINIC ACID, ... (4 entities in total)
• 機能のキーワード: aminotransferase, transaminase, amines synthesis, enantioselective, thermostable, engineered, transferase
• 由来する生物種: Pseudomonas sp.
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 101863.98

構造登録者

Capra, N.,Rozeboom, H.J.,Thunnissen, A.M.W.H.,Janssen, D.B. (登録日: 2020-12-02, 公開日: 2021-09-01, 最終更新日: 2024-06-19)

主引用文献

Meng, Q.,Ramirez-Palacios, C.,Capra, N.,Hooghwinkel, M.E.,Thallmair, S.,Rozeboom, H.J.,Thunnissen, A.W.H.,Wijma, H.J.,Marrink, S.J.,Janssen, D.B.
Computational Redesign of an omega-Transaminase from Pseudomonas jessenii for Asymmetric Synthesis of Enantiopure Bulky Amines.
Acs Catalysis, 11:10733-10747, 2021

PubMed Abstract: ω-Transaminases (ω-TA) are attractive biocatalysts for the production of chiral amines from prochiral ketones asymmetric synthesis. However, the substrate scope of ω-TAs is usually limited due to steric hindrance at the active site pockets. We explored a protein engineering strategy using computational design to expand the substrate scope of an ()-selective ω-TA from (TA-R6) toward the production of bulky amines. TA-R6 is attractive for use in applied biocatalysis due to its thermostability, tolerance to organic solvents, and acceptance of high concentrations of isopropylamine as amino donor. TA-R6 showed no detectable activity for the synthesis of six bicyclic or bulky amines targeted in this study. Six small libraries composed of 7-18 variants each were separately designed computational methods and tested in the laboratory for ketone to amine conversion. In each library, the vast majority of the variants displayed the desired activity, and of the 40 different designs, 38 produced the target amine in good yield with >99% enantiomeric excess. This shows that the substrate scope and enantioselectivity of TA mutants could be predicted with high accuracy. The single mutant W58G showed the best performance in the synthesis of five structurally similar bulky amines containing the indan and tetralin moieties. The best variant for the other bulky amine, 1-phenylbutylamine, was the triple mutant W58M + F86L + R417L, indicating that Trp58 is a key residue in the large binding pocket for TA-R6 redesign. Crystal structures of the two best variants confirmed the computationally predicted structures. The results show that computational design can be an efficient approach to rapidly expand the substrate scope of ω-TAs to produce enantiopure bulky amines.

PubMed: 34504735
DOI: 10.1021/acscatal.1c02053

実験手法

X-RAY DIFFRACTION (1.9 Å)