7AYV

X-ray crystallographic structure of (6-4)photolyase from Drosophila melanogaster at cryogenic temperature

7AYV の概要

• エントリーDOI: 10.2210/pdb7ayv/pdb
• 分子名称: RE11660p, GLYCEROL, FLAVIN-ADENINE DINUCLEOTIDE, ... (5 entities in total)
• 機能のキーワード: flavoprotein, (6-4)photolyase, lyase
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 59671.00

構造登録者

Cellini, A.,Wahlgren, W.Y.,Henry, L.,Westenhoff, S. (登録日: 2020-11-13, 公開日: 2021-08-18, 最終更新日: 2024-01-31)

主引用文献

Cellini, A.,Yuan Wahlgren, W.,Henry, L.,Pandey, S.,Ghosh, S.,Castillon, L.,Claesson, E.,Takala, H.,Kubel, J.,Nimmrich, A.,Kuznetsova, V.,Nango, E.,Iwata, S.,Owada, S.,Stojkovic, E.A.,Schmidt, M.,Ihalainen, J.A.,Westenhoff, S.
The three-dimensional structure of Drosophila melanogaster (6-4) photolyase at room temperature.
Acta Crystallogr D Struct Biol, 77:1001-1009, 2021

PubMed Abstract: (6-4) photolyases are flavoproteins that belong to the photolyase/cryptochrome family. Their function is to repair DNA lesions using visible light. Here, crystal structures of Drosophila melanogaster (6-4) photolyase [Dm(6-4)photolyase] at room and cryogenic temperatures are reported. The room-temperature structure was solved to 2.27 Å resolution and was obtained by serial femtosecond crystallography (SFX) using an X-ray free-electron laser. The crystallization and preparation conditions are also reported. The cryogenic structure was solved to 1.79 Å resolution using conventional X-ray crystallography. The structures agree with each other, indicating that the structural information obtained from crystallography at cryogenic temperature also applies at room temperature. Furthermore, UV-Vis absorption spectroscopy confirms that Dm(6-4)photolyase is photoactive in the crystals, giving a green light to time-resolved SFX studies on the protein, which can reveal the structural mechanism of the photoactivated protein in DNA repair.

PubMed: 34342273
DOI: 10.1107/S2059798321005830

実験手法

X-RAY DIFFRACTION (1.79 Å)