7AV6

FAST in a domain-swapped dimer form

7AV6 の概要

• エントリーDOI: 10.2210/pdb7av6/pdb
• 分子名称: Photoactive yellow protein, FORMIC ACID (3 entities in total)
• 機能のキーワード: fluorescence, fluorogen, fluorogen-activating protein, fluorescent labelling, fast, fluorescent protein
• 由来する生物種: Halorhodospira halophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15341.22

構造登録者

Bukhdruker, S.,Remeeva, A.,Ruchkin, D.,Gorbachev, D.,Povarova, N.,Mineev, K.,Goncharuk, S.,Baranov, M.,Mishin, A.,Borshchevskiy, V. (登録日: 2020-11-04, 公開日: 2021-06-09, 最終更新日: 2024-01-31)

主引用文献

Mineev, K.S.,Goncharuk, S.A.,Goncharuk, M.V.,Povarova, N.V.,Sokolov, A.I.,Baleeva, N.S.,Smirnov, A.Y.,Myasnyanko, I.N.,Ruchkin, D.A.,Bukhdruker, S.,Remeeva, A.,Mishin, A.,Borshchevskiy, V.,Gordeliy, V.,Arseniev, A.S.,Gorbachev, D.A.,Gavrikov, A.S.,Mishin, A.S.,Baranov, M.S.
NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids.
Chem Sci, 12:6719-6725, 2021

PubMed Abstract: One of the essential characteristics of any tag used in bioscience and medical applications is its size. The larger the label, the more it may affect the studied object, and the more it may distort its behavior. In this paper, using NMR spectroscopy and X-ray crystallography, we have studied the structure of fluorogen-activating protein FAST both in the apo form and in complex with the fluorogen. We showed that significant change in the protein occurs upon interaction with the ligand. While the protein is completely ordered in the complex, its apo form is characterized by higher mobility and disordering of its N-terminus. We used structural information to design the shortened FAST (which we named nanoFAST) by truncating 26 N-terminal residues. Thus, we created the shortest genetically encoded tag among all known fluorescent and fluorogen-activating proteins, which is composed of only 98 amino acids.

PubMed: 34040747
DOI: 10.1039/d1sc01454d

実験手法

X-RAY DIFFRACTION (1.5 Å)