7AV4

Dark state structure of the C432S mutant of Fatty Acid Photodecarboxylase (FAP)

これはPDB形式変換不可エントリーです。

7AV4 の概要

• エントリーDOI: 10.2210/pdb7av4/pdb
• 関連するPDBエントリー: 6yru 6yrv 6yrx 6yrz 6ys1 6ys2
• 分子名称: Fatty acid photodecarboxylase, chloroplastic, STEARIC ACID, FLAVIN-ADENINE DINUCLEOTIDE, ... (5 entities in total)
• 機能のキーワード: active site mutation dark state, oxidoreductase
• 由来する生物種: Chlorella variabilis (Green alga)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 70427.15

構造登録者

Schlichting, I.,Hartmann, E.,Arnoux, P.,Sorigue, D.,Beisson, F. (登録日: 2020-11-04, 公開日: 2021-04-21, 最終更新日: 2024-01-31)

主引用文献

Sorigue, D.,Hadjidemetriou, K.,Blangy, S.,Gotthard, G.,Bonvalet, A.,Coquelle, N.,Samire, P.,Aleksandrov, A.,Antonucci, L.,Benachir, A.,Boutet, S.,Byrdin, M.,Cammarata, M.,Carbajo, S.,Cuine, S.,Doak, R.B.,Foucar, L.,Gorel, A.,Grunbein, M.,Hartmann, E.,Hienerwadel, R.,Hilpert, M.,Kloos, M.,Lane, T.J.,Legeret, B.,Legrand, P.,Li-Beisson, Y.,Moulin, S.L.Y.,Nurizzo, D.,Peltier, G.,Schiro, G.,Shoeman, R.L.,Sliwa, M.,Solinas, X.,Zhuang, B.,Barends, T.R.M.,Colletier, J.P.,Joffre, M.,Royant, A.,Berthomieu, C.,Weik, M.,Domratcheva, T.,Brettel, K.,Vos, M.H.,Schlichting, I.,Arnoux, P.,Muller, P.,Beisson, F.
Mechanism and dynamics of fatty acid photodecarboxylase.
Science, 372:-, 2021

PubMed Abstract: Fatty acid photodecarboxylase (FAP) is a photoenzyme with potential green chemistry applications. By combining static, time-resolved, and cryotrapping spectroscopy and crystallography as well as computation, we characterized FAP reaction intermediates on time scales from subpicoseconds to milliseconds. High-resolution crystal structures from synchrotron and free electron laser x-ray sources highlighted an unusual bent shape of the oxidized flavin chromophore. We demonstrate that decarboxylation occurs directly upon reduction of the excited flavin by the fatty acid substrate. Along with flavin reoxidation by the alkyl radical intermediate, a major fraction of the cleaved carbon dioxide unexpectedly transformed in 100 nanoseconds, most likely into bicarbonate. This reaction is orders of magnitude faster than in solution. Two strictly conserved residues, R451 and C432, are essential for substrate stabilization and functional charge transfer.

PubMed: 33833098
DOI: 10.1126/science.abd5687

実験手法

X-RAY DIFFRACTION (1.936 Å)