7ABW

Crystal structure of siderophore reductase FoxB

7ABW の概要

• エントリーDOI: 10.2210/pdb7abw/pdb
• 分子名称: PepSY domain-containing protein, PROTOPORPHYRIN IX CONTAINING FE, 3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL, ... (5 entities in total)
• 機能のキーワード: membrane protein reductase siderophore, membrane protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 91698.39

構造登録者

Josts, I.,Tidow, H. (登録日: 2020-09-09, 公開日: 2021-09-01, 最終更新日: 2024-06-19)

主引用文献

Josts, I.,Veith, K.,Normant, V.,Schalk, I.J.,Tidow, H.
Structural insights into a novel family of integral membrane siderophore reductases.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: Gram-negative bacteria take up the essential ion Fe as ferric-siderophore complexes through their outer membrane using TonB-dependent transporters. However, the subsequent route through the inner membrane differs across many bacterial species and siderophore chemistries and is not understood in detail. Here, we report the crystal structure of the inner membrane protein FoxB (from ) that is involved in Fe-siderophore uptake. The structure revealed a fold with two tightly bound heme molecules. In combination with in vitro reduction assays and in vivo iron uptake studies, these results establish FoxB as an inner membrane reductase involved in the release of iron from ferrioxamine during Fe-siderophore uptake.

PubMed: 34417315
DOI: 10.1073/pnas.2101952118

実験手法

X-RAY DIFFRACTION (3.35 Å)