7ABH

Human pre-Bact-2 spliceosome (SF3b/U2 snRNP portion)

7ABH の概要

• エントリーDOI: 10.2210/pdb7abh/pdb
• 関連するPDBエントリー: 7AAV
• EMDBエントリー: 11693 11696
• 分子名称: Cell division cycle 5-like protein, Splicing factor 3B subunit 5, Splicing factor 3B subunit 6, ... (16 entities in total)
• 機能のキーワード: complex, spliceosome, catalytic activation, splicing
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 1029670.91

構造登録者

Townsend, C.,Kastner, B.,Leelaram, M.N.,Bertram, K.,Stark, H.,Luehrmann, R. (登録日: 2020-09-07, 公開日: 2020-12-09, 最終更新日: 2024-05-01)

主引用文献

Townsend, C.,Leelaram, M.N.,Agafonov, D.E.,Dybkov, O.,Will, C.L.,Bertram, K.,Urlaub, H.,Kastner, B.,Stark, H.,Luhrmann, R.
Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation.
Science, 370:-, 2020

PubMed Abstract: Spliceosome activation involves extensive protein and RNA rearrangements that lead to formation of a catalytically active U2/U6 RNA structure. At present, little is known about the assembly pathway of the latter and the mechanism whereby proteins aid its proper folding. Here, we report the cryo-electron microscopy structures of two human, activated spliceosome precursors (that is, pre-B complexes) at core resolutions of 3.9 and 4.2 angstroms. These structures elucidate the order of the numerous protein exchanges that occur during activation, the mutually exclusive interactions that ensure the correct order of ribonucleoprotein rearrangements needed to form the U2/U6 catalytic RNA, and the stepwise folding pathway of the latter. Structural comparisons with mature B complexes reveal the molecular mechanism whereby a conformational change in the scaffold protein PRP8 facilitates final three-dimensional folding of the U2/U6 catalytic RNA.

PubMed: 33243851
DOI: 10.1126/science.abc3753

実験手法

ELECTRON MICROSCOPY (4.5 Å)