7A7N

rsGreen0.7b in the green-off state

Summary for 7A7N

• Entry DOI: 10.2210/pdb7a7n/pdb
• Related: 4XOV 4XOW
• Descriptor: Green fluorescent protein (2 entities in total)
• Functional Keywords: reversible photoswitchable fluorescent protein, fluorescent protein
• Biological source: Aequorea victoria (Jellyfish)
• Total number of polymer chains: 1
• Total formula weight: 30685.44

Authors

De Zitter, E.,Dedecker, P.,Van Meervelt, L. (deposition date: 2020-08-30, release date: 2021-02-17, Last modification date: 2023-11-15)

Primary citation

De Zitter, E.,Hugelier, S.,Duwe, S.,Vandenberg, W.,Tebo, A.G.,Van Meervelt, L.,Dedecker, P.
Structure-Function Dataset Reveals Environment Effects within a Fluorescent Protein Model System*.
Angew.Chem.Int.Ed.Engl., 60:10073-10081, 2021

PubMed Abstract: Anisotropic environments can drastically alter the spectroscopy and photochemistry of molecules, leading to complex structure-function relationships. We examined this using fluorescent proteins as easy-to-modify model systems. Starting from a single scaffold, we have developed a range of 27 photochromic fluorescent proteins that cover a broad range of spectroscopic properties, including the determination of 43 crystal structures. Correlation and principal component analysis confirmed the complex relationship between structure and spectroscopy, but also allowed us to identify consistent trends and to relate these to the spatial organization. We find that changes in spectroscopic properties can come about through multiple underlying mechanisms, of which polarity, hydrogen bonding and presence of water molecules are key modulators. We anticipate that our findings and rich structure/spectroscopy dataset can open opportunities for the development and evaluation of new and existing protein engineering methods.

PubMed: 33543524
DOI: 10.1002/anie.202015201

Experimental method

X-RAY DIFFRACTION (1.2 Å)