7A62

Structure of human indoleamine-2,3-dioxygenase 1 (hIDO1) with a complete JK loop

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7A62 の概要

• エントリーDOI: 10.2210/pdb7a62/pdb
• 分子名称: Indoleamine 2,3-dioxygenase 1, GLYCEROL, PROTOPORPHYRIN IX CONTAINING FE, ... (5 entities in total)
• 機能のキーワード: dioxygenase, tryptophan catabolism, heme-binding, enzyme, oxidoreductase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 184385.10

構造登録者

Mirgaux, M.,Wouters, J. (登録日: 2020-08-24, 公開日: 2020-12-16, 最終更新日: 2024-01-31)

主引用文献

Mirgaux, M.,Leherte, L.,Wouters, J.
Influence of the presence of the heme cofactor on the JK-loop structure in indoleamine 2,3-dioxygenase 1.
Acta Crystallogr D Struct Biol, 76:1211-1221, 2020

PubMed Abstract: Indoleamine 2,3-dioxygenase 1 has sparked interest as an immunotherapeutic target in cancer research. Its structure includes a loop, named the JK-loop, that controls the orientation of the substrate or inhibitor within the active site. However, little has been reported about the crystal structure of this loop. In the present work, the conformation of the JK-loop is determined for the first time in the presence of the heme cofactor in the active site through X-ray diffraction experiments (2.44 Å resolution). Molecular-dynamics trajectories were also obtained to provide dynamic information about the loop according to the presence of cofactor. This new structural and dynamic information highlights the importance of the JK-loop in confining the labile heme cofactor to the active site.

PubMed: 33263327
DOI: 10.1107/S2059798320013510

実験手法

X-RAY DIFFRACTION (2.43796448682 Å)