7A01
The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism
This is a non-PDB format compatible entry.
Summary for 7A01
Entry DOI | 10.2210/pdb7a01/pdb |
Related | 6ZVK |
EMDB information | 11459 11590 |
Descriptor | INTERNAL RIBOSOME ENTRY SITE, uL15, 60S ribosomal protein L29, ... (86 entities in total) |
Functional Keywords | dicistrovirus halastavi arva virus intergenic region internal ribosome entry site ires rna pseudoknot ribosome serbp1 crpv igr ires, ribosome |
Biological source | Halastavi arva RNA virus More |
Total number of polymer chains | 85 |
Total formula weight | 3332086.40 |
Authors | Abaeva, I.,Vicens, Q.,Bochler, A.,Soufari, H.,Simonetti, A.,Pestova, T.V.,Hashem, Y.,Hellen, C.U.T. (deposition date: 2020-08-05, release date: 2020-12-30) |
Primary citation | Abaeva, I.S.,Vicens, Q.,Bochler, A.,Soufari, H.,Simonetti, A.,Pestova, T.V.,Hashem, Y.,Hellen, C.U.T. The Halastavi arva Virus Intergenic Region IRES Promotes Translation by the Simplest Possible Initiation Mechanism. Cell Rep, 33:108476-108476, 2020 Cited by PubMed Abstract: Dicistrovirus intergenic region internal ribosomal entry sites (IGR IRESs) do not require initiator tRNA, an AUG codon, or initiation factors and jumpstart translation from the middle of the elongation cycle via formation of IRES/80S complexes resembling the pre-translocation state. eEF2 then translocates the [codon-anticodon]-mimicking pseudoknot I (PKI) from ribosomal A sites to P sites, bringing the first sense codon into the decoding center. Halastavi árva virus (HalV) contains an IGR that is related to previously described IGR IRESs but lacks domain 2, which enables these IRESs to bind to individual 40S ribosomal subunits. By using in vitro reconstitution and cryoelectron microscopy (cryo-EM), we now report that the HalV IGR IRES functions by the simplest initiation mechanism that involves binding to 80S ribosomes such that PKI is placed in the P site, so that the A site contains the first codon that is directly accessible for decoding without prior eEF2-mediated translocation of PKI. PubMed: 33296660DOI: 10.1016/j.celrep.2020.108476 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.6 Å) |
Structure validation
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