7A01

The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism

これはPDB形式変換不可エントリーです。

7A01 の概要

• エントリーDOI: 10.2210/pdb7a01/pdb
• 関連するPDBエントリー: 6ZVK
• EMDBエントリー: 11459 11590
• 分子名称: INTERNAL RIBOSOME ENTRY SITE, uL15, 60S ribosomal protein L29, ... (86 entities in total)
• 機能のキーワード: dicistrovirus halastavi arva virus intergenic region internal ribosome entry site ires rna pseudoknot ribosome serbp1 crpv igr ires, ribosome
• 由来する生物種: Halastavi arva RNA virus; 詳細
• タンパク質・核酸の鎖数: 85
• 化学式量合計: 3332086.40

構造登録者

Abaeva, I.,Vicens, Q.,Bochler, A.,Soufari, H.,Simonetti, A.,Pestova, T.V.,Hashem, Y.,Hellen, C.U.T. (登録日: 2020-08-05, 公開日: 2020-12-30, 最終更新日: 2025-12-17)

主引用文献

Abaeva, I.S.,Vicens, Q.,Bochler, A.,Soufari, H.,Simonetti, A.,Pestova, T.V.,Hashem, Y.,Hellen, C.U.T.
The Halastavi arva Virus Intergenic Region IRES Promotes Translation by the Simplest Possible Initiation Mechanism.
Cell Rep, 33:108476-108476, 2020

PubMed Abstract: Dicistrovirus intergenic region internal ribosomal entry sites (IGR IRESs) do not require initiator tRNA, an AUG codon, or initiation factors and jumpstart translation from the middle of the elongation cycle via formation of IRES/80S complexes resembling the pre-translocation state. eEF2 then translocates the [codon-anticodon]-mimicking pseudoknot I (PKI) from ribosomal A sites to P sites, bringing the first sense codon into the decoding center. Halastavi árva virus (HalV) contains an IGR that is related to previously described IGR IRESs but lacks domain 2, which enables these IRESs to bind to individual 40S ribosomal subunits. By using in vitro reconstitution and cryoelectron microscopy (cryo-EM), we now report that the HalV IGR IRES functions by the simplest initiation mechanism that involves binding to 80S ribosomes such that PKI is placed in the P site, so that the A site contains the first codon that is directly accessible for decoding without prior eEF2-mediated translocation of PKI.

PubMed: 33296660
DOI: 10.1016/j.celrep.2020.108476

実験手法

ELECTRON MICROSCOPY (3.6 Å)