7VY3
STRUCTURE OF PHOTOSYNTHETIC LH1-RC SUPER-COMPLEX OF RHODOBACTER SPHAEROIDES LACKING PROTEIN-U
Summary for 7VY3
| Entry DOI | 10.2210/pdb7vy3/pdb |
| EMDB information | 32193 |
| Descriptor | Photosynthetic reaction center L subunit, (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE, LAURYL DIMETHYLAMINE-N-OXIDE, ... (16 entities in total) |
| Functional Keywords | lh1-rc complex, photosynthesis, purple bacteria |
| Biological source | Rhodobacter sphaeroides f. sp. denitrificans More |
| Total number of polymer chains | 25 |
| Total formula weight | 288908.91 |
| Authors | Tani, K.,Kanno, R.,Kawamura, S.,Kikuchi, R.,Nagashima, K.V.P.,Hall, M.,Takahashi, A.,Yu, L.-J.,Kimura, Y.,Madigan, M.T.,Mizoguchi, A.,Humbel, B.M.,Wang-Otomo, Z.-Y. (deposition date: 2021-11-13, release date: 2022-04-27, Last modification date: 2024-11-13) |
| Primary citation | Tani, K.,Kanno, R.,Kikuchi, R.,Kawamura, S.,Nagashima, K.V.P.,Hall, M.,Takahashi, A.,Yu, L.J.,Kimura, Y.,Madigan, M.T.,Mizoguchi, A.,Humbel, B.M.,Wang-Otomo, Z.Y. Asymmetric structure of the native Rhodobacter sphaeroides dimeric LH1-RC complex. Nat Commun, 13:1904-1904, 2022 Cited by PubMed Abstract: Rhodobacter sphaeroides is a model organism in bacterial photosynthesis, and its light-harvesting-reaction center (LH1-RC) complex contains both dimeric and monomeric forms. Here we present cryo-EM structures of the native LH1-RC dimer and an LH1-RC monomer lacking protein-U (ΔU). The native dimer reveals several asymmetric features including the arrangement of its two monomeric components, the structural integrity of protein-U, the overall organization of LH1, and rigidities of the proteins and pigments. PufX plays a critical role in connecting the two monomers in a dimer, with one PufX interacting at its N-terminus with another PufX and an LH1 β-polypeptide in the other monomer. One protein-U was only partially resolved in the dimeric structure, signaling different degrees of disorder in the two monomers. The ΔU LH1-RC monomer was half-moon-shaped and contained 11 α- and 10 β-polypeptides, indicating a critical role for protein-U in controlling the number of αβ-subunits required for dimer assembly and stabilization. These features are discussed in relation to membrane topology and an assembly model proposed for the native dimeric complex. PubMed: 35393413DOI: 10.1038/s41467-022-29453-8 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.63 Å) |
Structure validation
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