7UKJ
In situ cryo-EM structure of bacteriophage Sf6 portal:gp7 complex at 2.7A resolution
Summary for 7UKJ
| Entry DOI | 10.2210/pdb7ukj/pdb |
| EMDB information | 26582 |
| Descriptor | Gene 14 protein (1 entity in total) |
| Functional Keywords | in situ, phage, portal, gp7, gp3, structural protein, viral protein |
| Biological source | Shigella phage Sf6 |
| Total number of polymer chains | 3 |
| Total formula weight | 201354.42 |
| Authors | Li, F.,Cingolani, G.,Hou, C. (deposition date: 2022-04-01, release date: 2022-12-21, Last modification date: 2024-06-12) |
| Primary citation | Li, F.,Hou, C.D.,Yang, R.,Whitehead III, R.,Teschke, C.M.,Cingolani, G. High-resolution cryo-EM structure of the Shigella virus Sf6 genome delivery tail machine. Sci Adv, 8:eadc9641-eadc9641, 2022 Cited by PubMed Abstract: Sf6 is a bacterial virus that infects the human pathogen Here, we describe the cryo-electron microscopy structure of the Sf6 tail machine before DNA ejection, which we determined at a 2.7-angstrom resolution. We built de novo structures of all tail components and resolved four symmetry-mismatched interfaces. Unexpectedly, we found that the tail exists in two conformations, rotated by ~6° with respect to the capsid. The two tail conformers are identical in structure but differ solely in how the portal and head-to-tail adaptor carboxyl termini bond with the capsid at the fivefold vertex, similar to a diamond held over a five-pronged ring in two nonidentical states. Thus, in the mature Sf6 tail, the portal structure does not morph locally to accommodate the symmetry mismatch but exists in two energetic minima rotated by a discrete angle. We propose that the design principles of the Sf6 tail are conserved across P22-like Podoviridae. PubMed: 36475795DOI: 10.1126/sciadv.adc9641 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.6 Å) |
Structure validation
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