7RM8

Solution NMR structure of PDLIM7 PDZ bound to SNX17 peptide

Summary for 7RM8

• Entry DOI: 10.2210/pdb7rm8/pdb
• NMR Information: BMRB: 30937
• Descriptor: Isoform 4 of PDZ and LIM domain protein 7,Sorting nexin-17 fusion (1 entity in total)
• Functional Keywords: structural protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 1
• Total formula weight: 11317.57

Authors

Healy, M.D.,Collins, B.M.,Mobli, M. (deposition date: 2021-07-26, release date: 2022-08-31, Last modification date: 2024-05-15)

Primary citation

Healy, M.D.,Sacharz, J.,McNally, K.E.,McConville, C.,Tillu, V.A.,Hall, R.J.,Chilton, M.,Cullen, P.J.,Mobli, M.,Ghai, R.,Stroud, D.A.,Collins, B.M.
Proteomic identification and structural basis for the interaction between sorting nexin SNX17 and PDLIM family proteins.
Structure, 30:1590-1602.e6, 2022

PubMed Abstract: The sorting nexin SNX17 controls endosomal recycling of transmembrane cargo proteins including integrins, the amyloid precursor protein, and lipoprotein receptors. This requires association with the Commander trafficking complex and depends on the C terminus of SNX17 through unknown mechanisms. Using proteomics, we find that the SNX17 C terminus is sufficient for Commander interaction and also associates with members of the PDZ and LIM domain (PDLIM) family. SNX17 contains a type III PDZ binding motif that binds specifically to the PDLIM proteins. The structure of the PDLIM7 PDZ domain bound to the SNX17 C terminus reveals an unconventional perpendicular peptide interaction mediated by electrostatic contacts and a uniquely conserved proline-containing loop sequence in the PDLIM protein family. Our results define the mechanism of SNX17-PDLIM interaction and suggest that the PDLIM proteins may play a role in regulating the activity of SNX17 in conjunction with Commander and actin-rich endosomal trafficking domains.

PubMed: 36302387
DOI: 10.1016/j.str.2022.10.001

Experimental method

SOLUTION NMR