7PKS

Structural basis of Integrator-mediated transcription regulation

This is a non-PDB format compatible entry.

Summary for 7PKS

• Entry DOI: 10.2210/pdb7pks/pdb
• EMDB information: 13479
• Descriptor: DNA-directed RNA polymerase subunit, DNA-directed RNA polymerases I, II, and III subunit RPABC5, RNA_pol_L_2 domain-containing protein, ... (36 entities in total)
• Functional Keywords: promoter proximal pausing, attenuation, snrna termination, integrator complex, transcription
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 33
• Total formula weight: 2033399.18

Authors

Fianu, I.,Chen, Y.,Dienemann, C.,Cramer, P. (deposition date: 2021-08-26, release date: 2021-12-08, Last modification date: 2024-10-09)

Primary citation

Fianu, I.,Chen, Y.,Dienemann, C.,Dybkov, O.,Linden, A.,Urlaub, H.,Cramer, P.
Structural basis of Integrator-mediated transcription regulation.
Science, 374:883-887, 2021

PubMed Abstract: Integrator and protein phosphatase 2A (PP2A) form a complex that dephosphorylates paused RNA polymerase II (Pol II), cleaves the nascent RNA, and terminates transcription. We report the structure of the pretermination complex containing the human Integrator-PP2A complex bound to paused Pol II. Integrator binds Pol II and the pausing factors DSIF and NELF to exclude binding of the elongation factors SPT6 and PAF1 complex. Integrator also binds the C-terminal domain of Pol II and positions PP2A to counteract Pol II phosphorylation and elongation. The Integrator endonuclease docks to the RNA exit site and opens to cleave nascent RNA about 20 nucleotides from the Pol II active site. Integrator does not bind the DNA clamps formed by Pol II and DSIF, enabling release of DNA and transcription termination.

PubMed: 34762484
DOI: 10.1126/science.abk0154

Experimental method

ELECTRON MICROSCOPY (3.6 Å)