7N8I
SARS-CoV-2 S (B.1.429 / epsilon variant) + S2M11 + S2L20 (Local Refinement of the NTD/S2L20)
Summary for 7N8I
| Entry DOI | 10.2210/pdb7n8i/pdb |
| EMDB information | 24236 24237 |
| Descriptor | S2L20 Fab Light Chain Variable Region, S2L20 Fab Heavy Chain Variable Region, Spike glycoprotein, ... (4 entities in total) |
| Functional Keywords | coronavirus, antibody, california, structural genomics, seattle structural genomics center for infectious disease, ssgcid, viral protein, viral protein-immune system complex, viral protein/immune system |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 3 |
| Total formula weight | 167744.57 |
| Authors | McCallum, M.,Seattle Structural Genomics Center for Infectious Disease (SSGCID),Veesler, D. (deposition date: 2021-06-14, release date: 2021-07-14, Last modification date: 2024-10-23) |
| Primary citation | McCallum, M.,Bassi, J.,De Marco, A.,Chen, A.,Walls, A.C.,Di Iulio, J.,Tortorici, M.A.,Navarro, M.J.,Silacci-Fregni, C.,Saliba, C.,Sprouse, K.R.,Agostini, M.,Pinto, D.,Culap, K.,Bianchi, S.,Jaconi, S.,Cameroni, E.,Bowen, J.E.,Tilles, S.W.,Pizzuto, M.S.,Guastalla, S.B.,Bona, G.,Pellanda, A.F.,Garzoni, C.,Van Voorhis, W.C.,Rosen, L.E.,Snell, G.,Telenti, A.,Virgin, H.W.,Piccoli, L.,Corti, D.,Veesler, D. SARS-CoV-2 immune evasion by the B.1.427/B.1.429 variant of concern. Science, 373:648-654, 2021 Cited by PubMed Abstract: A novel variant of concern (VOC) named CAL.20C (B.1.427/B.1.429), which was originally detected in California, carries spike glycoprotein mutations S13I in the signal peptide, W152C in the N-terminal domain (NTD), and L452R in the receptor-binding domain (RBD). Plasma from individuals vaccinated with a Wuhan-1 isolate-based messenger RNA vaccine or from convalescent individuals exhibited neutralizing titers that were reduced 2- to 3.5-fold against the B.1.427/B.1.429 variant relative to wild-type pseudoviruses. The L452R mutation reduced neutralizing activity in 14 of 34 RBD-specific monoclonal antibodies (mAbs). The S13I and W152C mutations resulted in total loss of neutralization for 10 of 10 NTD-specific mAbs because the NTD antigenic supersite was remodeled by a shift of the signal peptide cleavage site and the formation of a new disulfide bond, as revealed by mass spectrometry and structural studies. PubMed: 34210893DOI: 10.1126/science.abi7994 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3 Å) |
Structure validation
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