7N2W

The crystal structure of an FMN-dependent NADH-azoreductase, AzoA in complex with Red 40

Summary for 7N2W

• Entry DOI: 10.2210/pdb7n2w/pdb
• Related: 6DXP
• Descriptor: FMN dependent NADH:quinone oxidoreductase, FLAVIN MONONUCLEOTIDE, 6-hydroxy-5-[(E)-(2-methoxy-5-methyl-4-sulfophenyl)diazenyl]naphthalene-2-sulfonic acid, ... (4 entities in total)
• Functional Keywords: fmn, nadh, azoreductase, azoa, oxidoreductase
• Biological source: Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
• Total number of polymer chains: 4
• Total formula weight: 100665.26

Authors

Arcinas, A.J.,Fedorov, E.,Kelly, L.,Almo, S.C.,Ghosh, A. (deposition date: 2021-05-30, release date: 2022-06-01, Last modification date: 2026-10-28)

Primary citation

Hitchings, R.,Ghosh, A.,Arcinas, A.J.,Almo, S.C.,Kelly, L.,Ryan, A.
Ligand Binding Remodels the Oligomeric State and Active Site of Klebsiella pneumoniae Azoreductase.
Biochemistry, 65:3236-3246, 2026

PubMed Abstract: Azo dyes are widespread in the environment, and humans ingest a range of these dyes present in food and drugs. The human gut microbiome significantly impacts the fate of ingested azo dyes primarily through the reduction of the azo bond (R-N = N-R'). Flavin-dependent azoreductases have been considered the key enzymatic drivers of this process. To challenge this paradigm, we constructed a sequence similarity network of known azoreductases and focused on AzoA of Klebsiella pneumoniae, a central representative within the gut microbiome cluster. Unexpectedly, K. pneumoniae AzoA displayed relatively low in vitro activity against a range of azo compounds. While the physiological implications remain unclear, these findings are consistent with the possibility that nonenzymatic mechanisms contribute substantially to azo compound reduction in the gut. Furthermore, structural characterization of AzoA, as a holoenzyme and in its ligand-bound state, revealed a previously unrecognized ligand-coupled mechanism involving remodeling of the active site and oligomeric state.

PubMed: 42899598
DOI: 10.1021/acs.biochem.6c00568

Experimental method

X-RAY DIFFRACTION (2.65 Å)