7MK7
Augmentor domain of augmentor-beta
Summary for 7MK7
| Entry DOI | 10.2210/pdb7mk7/pdb |
| Related PRD ID | PRD_900001 |
| Descriptor | ALK and LTK ligand 1,Maltodextrin-binding protein, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose (3 entities in total) |
| Functional Keywords | cell signaling, cytokine |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 2 |
| Total formula weight | 93926.41 |
| Authors | Krimmer, S.G.,Reshetnyak, A.V.,Puleo, D.E.,Schlessinger, J. (deposition date: 2021-04-21, release date: 2021-11-24, Last modification date: 2024-10-23) |
| Primary citation | Li, T.,Stayrook, S.E.,Tsutsui, Y.,Zhang, J.,Wang, Y.,Li, H.,Proffitt, A.,Krimmer, S.G.,Ahmed, M.,Belliveau, O.,Walker, I.X.,Mudumbi, K.C.,Suzuki, Y.,Lax, I.,Alvarado, D.,Lemmon, M.A.,Schlessinger, J.,Klein, D.E. Structural basis for ligand reception by anaplastic lymphoma kinase. Nature, 600:148-152, 2021 Cited by PubMed Abstract: The proto-oncogene ALK encodes anaplastic lymphoma kinase, a receptor tyrosine kinase that is expressed primarily in the developing nervous system. After development, ALK activity is associated with learning and memory and controls energy expenditure, and inhibition of ALK can prevent diet-induced obesity. Aberrant ALK signalling causes numerous cancers. In particular, full-length ALK is an important driver in paediatric neuroblastoma, in which it is either mutated or activated by ligand. Here we report crystal structures of the extracellular glycine-rich domain (GRD) of ALK, which regulates receptor activity by binding to activating peptides. Fusing the ALK GRD to its ligand enabled us to capture a dimeric receptor complex that reveals how ALK responds to its regulatory ligands. We show that repetitive glycines in the GRD form rigid helices that separate the major ligand-binding site from a distal polyglycine extension loop (PXL) that mediates ALK dimerization. The PXL of one receptor acts as a sensor for the complex by interacting with a ligand-bound second receptor. ALK activation can be abolished through PXL mutation or with PXL-targeting antibodies. Together, these results explain how ALK uses its atypical architecture for its regulation, and suggest new therapeutic opportunities for ALK-expressing cancers such as paediatric neuroblastoma. PubMed: 34819665DOI: 10.1038/s41586-021-04141-7 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.42815184462 Å) |
Structure validation
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