7JPT

Structure of an endocytic receptor

Summary for 7JPT

• Entry DOI: 10.2210/pdb7jpt/pdb
• EMDB information: 22422
• Descriptor: Lymphocyte antigen 75, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• Functional Keywords: cell-surface receptor, immune receptor, mannose receptor family, immune system
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 196259.21

Authors

Gully, B.S.,Rossjohn, J.,Berry, R. (deposition date: 2020-08-09, release date: 2020-12-09, Last modification date: 2024-11-20)

Primary citation

Gully, B.S.,Venugopal, H.,Fulcher, A.J.,Fu, Z.,Li, J.,Deuss, F.A.,Llerena, C.,Heath, W.R.,Lahoud, M.H.,Caminschi, I.,Rossjohn, J.,Berry, R.
The cryo-EM structure of the endocytic receptor DEC-205.
J.Biol.Chem., 296:100127-100127, 2020

PubMed Abstract: DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine oligonucleotides, a motif often seen in bacterial or viral DNA. However, despite growing biological and clinical significance, little is known about the structural arrangement of this receptor or any of its family members. Here, we describe the 3.2 Å cryo-EM structure of human DEC-205, thereby illuminating the structure of the mannose receptor protein family. The DEC-205 monomer forms a compact structure comprising two intercalated rings of C-type lectin-like domains, where the N-terminal cysteine-rich and fibronectin domains reside at the central intersection. We establish a pH-dependent oligomerization pathway forming tetrameric DEC-205 using solution-based techniques and ultimately solved the 4.9 Å cryo-EM structure of the DEC-205 tetramer to identify the unfurling of the second lectin ring which enables tetramer formation. Furthermore, we suggest the relevance of this oligomerization pathway within a cellular setting, whereby cytosine-guanosine binding appeared to disrupt this cell-surface oligomer. Accordingly, we provide insight into the structure and oligomeric assembly of the DEC-205 receptor.

PubMed: 33257321
DOI: 10.1074/jbc.RA120.016451

Experimental method

ELECTRON MICROSCOPY (3.2 Å)