7D1U

Cryo-EM Structure of PSII at 2.08 angstrom resolution

Summary for 7D1U

• Entry DOI: 10.2210/pdb7d1u/pdb
• EMDB information: 30548
• Descriptor: Photosystem II protein D1, Photosystem II reaction center protein K, Photosystem II reaction center protein L, ... (39 entities in total)
• Functional Keywords: photosystem ii, photosynthesis
• Biological source: Thermosynechococcus vulcanus; More
• Total number of polymer chains: 40
• Total formula weight: 709085.07

Authors

Kato, K.,Miyazaki, N.,Hamaguchi, T.,Nakajima, Y.,Akita, F.,Yonekura, K.,Shen, J.R. (deposition date: 2020-09-15, release date: 2021-03-31, Last modification date: 2021-04-07)

Primary citation

Kato, K.,Miyazaki, N.,Hamaguchi, T.,Nakajima, Y.,Akita, F.,Yonekura, K.,Shen, J.R.
High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams.
Commun Biol, 4:382-382, 2021

PubMed Abstract: Photosystem II (PSII) plays a key role in water-splitting and oxygen evolution. X-ray crystallography has revealed its atomic structure and some intermediate structures. However, these structures are in the crystalline state and its final state structure has not been solved. Here we analyzed the structure of PSII in solution at 1.95 Å resolution by single-particle cryo-electron microscopy (cryo-EM). The structure obtained is similar to the crystal structure, but a PsbY subunit was visible in the cryo-EM structure, indicating that it represents its physiological state more closely. Electron beam damage was observed at a high-dose in the regions that were easily affected by redox states, and reducing the beam dosage by reducing frames from 50 to 2 yielded a similar resolution but reduced the damage remarkably. This study will serve as a good indicator for determining damage-free cryo-EM structures of not only PSII but also all biological samples, especially redox-active metalloproteins.

PubMed: 33753866
DOI: 10.1038/s42003-021-01919-3

Experimental method

ELECTRON MICROSCOPY (2.08 Å)