7AVA
Solution structure of the fluorogen-activating protein FAST in complex with the ligand N871b
Summary for 7AVA
| Entry DOI | 10.2210/pdb7ava/pdb |
| NMR Information | BMRB: 34572 |
| Descriptor | FAST, (5~{Z})-5-[(2-methoxy-4-oxidanyl-phenyl)methylidene]-3-methyl-2-[(~{E})-2-pyridin-4-ylethenyl]imidazol-4-one (2 entities in total) |
| Functional Keywords | fluorogen-activating protein pas domain, fluorescent protein |
| Biological source | synthetic construct |
| Total number of polymer chains | 1 |
| Total formula weight | 15584.53 |
| Authors | Mineev, K.S.,Goncharuk, S.A.,Baranov, M.S. (deposition date: 2020-11-05, release date: 2021-04-28, Last modification date: 2024-06-19) |
| Primary citation | Mineev, K.S.,Goncharuk, S.A.,Goncharuk, M.V.,Povarova, N.V.,Sokolov, A.I.,Baleeva, N.S.,Smirnov, A.Y.,Myasnyanko, I.N.,Ruchkin, D.A.,Bukhdruker, S.,Remeeva, A.,Mishin, A.,Borshchevskiy, V.,Gordeliy, V.,Arseniev, A.S.,Gorbachev, D.A.,Gavrikov, A.S.,Mishin, A.S.,Baranov, M.S. NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids. Chem Sci, 12:6719-6725, 2021 Cited by PubMed Abstract: One of the essential characteristics of any tag used in bioscience and medical applications is its size. The larger the label, the more it may affect the studied object, and the more it may distort its behavior. In this paper, using NMR spectroscopy and X-ray crystallography, we have studied the structure of fluorogen-activating protein FAST both in the apo form and in complex with the fluorogen. We showed that significant change in the protein occurs upon interaction with the ligand. While the protein is completely ordered in the complex, its apo form is characterized by higher mobility and disordering of its N-terminus. We used structural information to design the shortened FAST (which we named nanoFAST) by truncating 26 N-terminal residues. Thus, we created the shortest genetically encoded tag among all known fluorescent and fluorogen-activating proteins, which is composed of only 98 amino acids. PubMed: 34040747DOI: 10.1039/d1sc01454d PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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