6ZZG

MB_CRS6-1 bound to CrSAS-6_N

6ZZG の概要

• エントリーDOI: 10.2210/pdb6zzg/pdb
• 分子名称: Centriole protein, MB_CRS6-15, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: centriole, cartwheel, ring-polymer, monobody, protein binding
• 由来する生物種: Chlamydomonas reinhardtii; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 167770.64

構造登録者

Hatzopoulos, G.N.,Kukenshoner, T.,Banterle, N.,Favez, T.,Fluckiger, I.,Hantschel, O.,Gonczy, P. (登録日: 2020-08-04, 公開日: 2021-07-07, 最終更新日: 2024-01-31)

主引用文献

Hatzopoulos, G.N.,Kukenshoner, T.,Banterle, N.,Favez, T.,Fluckiger, I.,Hamel, V.,Andany, S.,Fantner, G.E.,Hantschel, O.,Gonczy, P.
Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly.
Nat Commun, 12:3805-3805, 2021

PubMed Abstract: Centrioles are evolutionarily conserved multi-protein organelles essential for forming cilia and centrosomes. Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that scaffolds organelle formation. The importance of this architecture has been difficult to decipher notably because of the lack of precise tools to modulate the underlying assembly reaction. Here, we developed monobodies against Chlamydomonas reinhardtii SAS-6, characterizing three in detail with X-ray crystallography, atomic force microscopy and cryo-electron microscopy. This revealed distinct monobody-target interaction modes, as well as specific consequences on ring assembly and stacking. Of particular interest, monobody MB-15 induces a conformational change in CrSAS-6, resulting in the formation of a helix instead of a ring. Furthermore, we show that this alteration impairs centriole biogenesis in human cells. Overall, our findings identify monobodies as powerful molecular levers to alter the architecture of multi-protein complexes and tune centriole assembly.

PubMed: 34155202
DOI: 10.1038/s41467-021-23897-0

実験手法

X-RAY DIFFRACTION (2.93 Å)