6ZY0

Catabolic reductive dehalogenase NpRdhA, N-terminally tagged, K488Q variant

6ZY0 の概要

• エントリーDOI: 10.2210/pdb6zy0/pdb
• 分子名称: Oxidoreductase, NAD-binding/iron-sulfur cluster-binding protein, IRON/SULFUR CLUSTER, COBALAMIN, ... (6 entities in total)
• 機能のキーワード: reductive dehalogenase, b12, iron-sulfur, oxidoreductase
• 由来する生物種: Nitratireductor pacificus pht-3B
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 245405.33

構造登録者

Leys, D.,Halliwell, T. (登録日: 2020-07-30, 公開日: 2020-09-16, 最終更新日: 2024-01-31)

主引用文献

Halliwell, T.,Fisher, K.,Payne, K.A.P.,Rigby, S.E.J.,Leys, D.
Catabolic Reductive Dehalogenase Substrate Complex Structures Underpin Rational Repurposing of Substrate Scope.
Microorganisms, 8:-, 2020

PubMed Abstract: Reductive dehalogenases are responsible for the reductive cleavage of carbon-halogen bonds during organohalide respiration. A variety of mechanisms have been proposed for these cobalamin and [4Fe-4S] containing enzymes, including organocobalt, radical, or cobalt-halide adduct based catalysis. The latter was proposed for the oxygen-tolerant cataboli reductive dehalogenase (NpRdhA). Here, we present the first substrate bound NpRdhA crystal structures, confirming a direct cobalt-halogen interaction is established and providing a rationale for substrate preference. Product formation is observed due to X-ray photoreduction. Protein engineering enables rational alteration of substrate preference, providing a future blue print for the application of this and related enzymes in bioremediation.

PubMed: 32887524
DOI: 10.3390/microorganisms8091344

実験手法

X-RAY DIFFRACTION (2.13 Å)