6ZVK

The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism

This is a non-PDB format compatible entry.

Summary for 6ZVK

• Entry DOI: 10.2210/pdb6zvk/pdb
• EMDB information: 11459
• Descriptor: Ribosomal protein L14, uL3, 60S RIBOSOMAL PROTEIN EL20, ... (86 entities in total)
• Functional Keywords: ribosome internal ribosome entry site rna dicistrovirus halastavi arva virus intergenic region pseudoknot crpv igr ires serbp1, translation
• Biological source: Halastavi arva RNA virus; More
• Total number of polymer chains: 85
• Total formula weight: 3371248.14

Authors

Abaeva, I.S.,Vicens, Q.,Bochler, A.,Soufari, H.,Simonetti, A.,Pestova, T.,Hashem, Y.,Hellen, C.U.T. (deposition date: 2020-07-24, release date: 2020-12-23, Last modification date: 2024-05-01)

Primary citation

Abaeva, I.S.,Vicens, Q.,Bochler, A.,Soufari, H.,Simonetti, A.,Pestova, T.V.,Hashem, Y.,Hellen, C.U.T.
The Halastavi arva Virus Intergenic Region IRES Promotes Translation by the Simplest Possible Initiation Mechanism.
Cell Rep, 33:108476-108476, 2020

PubMed Abstract: Dicistrovirus intergenic region internal ribosomal entry sites (IGR IRESs) do not require initiator tRNA, an AUG codon, or initiation factors and jumpstart translation from the middle of the elongation cycle via formation of IRES/80S complexes resembling the pre-translocation state. eEF2 then translocates the [codon-anticodon]-mimicking pseudoknot I (PKI) from ribosomal A sites to P sites, bringing the first sense codon into the decoding center. Halastavi árva virus (HalV) contains an IGR that is related to previously described IGR IRESs but lacks domain 2, which enables these IRESs to bind to individual 40S ribosomal subunits. By using in vitro reconstitution and cryoelectron microscopy (cryo-EM), we now report that the HalV IGR IRES functions by the simplest initiation mechanism that involves binding to 80S ribosomes such that PKI is placed in the P site, so that the A site contains the first codon that is directly accessible for decoding without prior eEF2-mediated translocation of PKI.

PubMed: 33296660
DOI: 10.1016/j.celrep.2020.108476

Experimental method

ELECTRON MICROSCOPY (3.49 Å)