6ZTY

Assembly intermediates of orthoreovirus captured in the cell

6ZTY の概要

• エントリーDOI: 10.2210/pdb6zty/pdb
• EMDBエントリー: 22164
• 分子名称: Outer capsid protein mu-1, Outer capsid protein sigma-3 (2 entities in total)
• 機能のキーワード: assemble intermediates, orthoreovirus, cryo-electron tomography, cellular lamellae, virus like particle
• 由来する生物種: Reovirus sp.; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 331658.16

構造登録者

Sutton, G.C.,Stuart, D.I. (登録日: 2020-07-20, 公開日: 2020-09-16, 最終更新日: 2025-04-02)

主引用文献

Sutton, G.,Sun, D.,Fu, X.,Kotecha, A.,Hecksel, C.W.,Clare, D.K.,Zhang, P.,Stuart, D.I.,Boyce, M.
Assembly intermediates of orthoreovirus captured in the cell.
Nat Commun, 11:4445-4445, 2020

PubMed Abstract: Traditionally, molecular assembly pathways for viruses are inferred from high resolution structures of purified stable intermediates, low resolution images of cell sections and genetic approaches. Here, we directly visualise an unsuspected 'single shelled' intermediate for a mammalian orthoreovirus in cryo-preserved infected cells, by cryo-electron tomography of cellular lamellae. Particle classification and averaging yields structures to 5.6 Å resolution, sufficient to identify secondary structural elements and produce an atomic model of the intermediate, comprising 120 copies each of protein λ1 and σ2. This λ1 shell is 'collapsed' compared to the mature virions, with molecules pushed inwards at the icosahedral fivefolds by ~100 Å, reminiscent of the first assembly intermediate of certain prokaryotic dsRNA viruses. This supports the supposition that these viruses share a common ancestor, and suggests mechanisms for the assembly of viruses of the Reoviridae. Such methodology holds promise for dissecting the replication cycle of many viruses.

PubMed: 32895380
DOI: 10.1038/s41467-020-18243-9

実験手法

ELECTRON MICROSCOPY (5.6 Å)