6ZS0

Crystal structure of 5-dimethylallyltryptophan synthase from Streptomyces coelicolor

これはPDB形式変換不可エントリーです。

6ZS0 の概要

• エントリーDOI: 10.2210/pdb6zs0/pdb
• 関連するPDBエントリー: 6ZRX 6ZRY 6ZRZ
• 分子名称: DMATS type aromatic prenyltransferase, DI(HYDROXYETHYL)ETHER (3 entities in total)
• 機能のキーワード: complex, abba-barrel fold, prenyltransferase, transferase
• 由来する生物種: Streptomyces coelicolor
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 79579.41

構造登録者

Ostertag, E.,Broger, K.,Stehle, T.,Zocher, G. (登録日: 2020-07-15, 公開日: 2020-12-09, 最終更新日: 2024-01-31)

主引用文献

Ostertag, E.,Zheng, L.,Broger, K.,Stehle, T.,Li, S.M.,Zocher, G.
Reprogramming Substrate and Catalytic Promiscuity of Tryptophan Prenyltransferases.
J.Mol.Biol., 433:166726-166726, 2020

PubMed Abstract: Prenylation is a process widely prevalent in primary and secondary metabolism, contributing to functionality and chemical diversity in natural systems. Due to their high regio- and chemoselectivities, prenyltransferases are also valuable tools for creation of new compounds by chemoenzymatic synthesis and synthetic biology. Over the last ten years, biochemical and structural investigations shed light on the mechanism and key residues that control the catalytic process, but to date crucial information on how certain prenyltransferases control regioselectivity and chemoselectivity is still lacking. Here, we advance a general understanding of the enzyme family by contributing the first structure of a tryptophan C5-prenyltransferase 5-DMATS. Additinally, the structure of a bacterial tryptophan C6-prenyltransferase 6-DMATS was solved. Analysis and comparison of both substrate-bound complexes led to the identification of key residues for catalysis. Next, site-directed mutagenesis was successfully implemented to not only modify the prenyl donor specificity but also to redirect the prenylation, thereby switching the regioselectivity of 6-DMATS to that of 5-DMATS. The general strategy of structure-guided protein engineering should be applicable to other related prenyltransferases, thus enabling the production of novel prenylated compounds.

PubMed: 33249189
DOI: 10.1016/j.jmb.2020.11.025

実験手法

X-RAY DIFFRACTION (1.5 Å)