6ZOJ

SARS-CoV-2-Nsp1-40S complex, composite map

6ZOJ の概要

• エントリーDOI: 10.2210/pdb6zoj/pdb
• EMDBエントリー: 11320
• 分子名称: 18S ribosomal RNA, 40S ribosomal protein S8, 40S ribosomal protein S9, ... (38 entities in total)
• 機能のキーワード: inhibitor, mrna channel, 40s ribosomal subunit, translation
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV); 詳細
• タンパク質・核酸の鎖数: 36
• 化学式量合計: 1236182.05

構造登録者

Schubert, K.,Karousis, E.D.,Jomaa, A.,Scaiola, A.,Echeverria, B.,Gurzeler, L.-A.,Leibundgut, M.L.,Thiel, V.,Muehlemann, O.,Ban, N. (登録日: 2020-07-07, 公開日: 2020-07-22, 最終更新日: 2021-02-10)

主引用文献

Schubert, K.,Karousis, E.D.,Jomaa, A.,Scaiola, A.,Echeverria, B.,Gurzeler, L.A.,Leibundgut, M.,Thiel, V.,Muhlemann, O.,Ban, N.
SARS-CoV-2 Nsp1 binds the ribosomal mRNA channel to inhibit translation.
Nat.Struct.Mol.Biol., 27:959-966, 2020

PubMed Abstract: The SARS-CoV-2 non-structural protein 1 (Nsp1), also referred to as the host shutoff factor, suppresses host innate immune functions. By combining cryo-electron microscopy and biochemistry, we show that SARS-CoV-2 Nsp1 binds to the human 40S subunit in ribosomal complexes, including the 43S pre-initiation complex and the non-translating 80S ribosome. The protein inserts its C-terminal domain into the mRNA channel, where it interferes with mRNA binding. We observe translation inhibition in the presence of Nsp1 in an in vitro translation system and in human cells. Based on the high-resolution structure of the 40S-Nsp1 complex, we identify residues of Nsp1 crucial for mediating translation inhibition. We further show that the full-length 5' untranslated region of the genomic viral mRNA stimulates translation in vitro, suggesting that SARS-CoV-2 combines global inhibition of translation by Nsp1 with efficient translation of the viral mRNA to allow expression of viral genes.

PubMed: 32908316
DOI: 10.1038/s41594-020-0511-8

実験手法

ELECTRON MICROSCOPY (2.8 Å)