6ZOA

Partially induced AcrB T protomer and DDM binding to the TM8/PC2 pathway of AcrB L2 protomer

6ZOA の概要

• エントリーDOI: 10.2210/pdb6zoa/pdb
• 分子名称: Multidrug efflux pump subunit AcrB, GLYCEROL, N-OCTANE, ... (15 entities in total)
• 機能のキーワード: multidrug efflux pump, membrane protein, transport protein
• 由来する生物種: Escherichia coli K-12; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 388910.49

構造登録者

Tam, H.K.,Foong, W.E.,Pos, K.M. (登録日: 2020-07-07, 公開日: 2021-05-19, 最終更新日: 2024-01-31)

主引用文献

Tam, H.K.,Foong, W.E.,Oswald, C.,Herrmann, A.,Zeng, H.,Pos, K.M.
Allosteric drug transport mechanism of multidrug transporter AcrB.
Nat Commun, 12:3889-3889, 2021

PubMed Abstract: Gram-negative bacteria maintain an intrinsic resistance mechanism against entry of noxious compounds by utilizing highly efficient efflux pumps. The E. coli AcrAB-TolC drug efflux pump contains the inner membrane H/drug antiporter AcrB comprising three functionally interdependent protomers, cycling consecutively through the loose (L), tight (T) and open (O) state during cooperative catalysis. Here, we present 13 X-ray structures of AcrB in intermediate states of the transport cycle. Structure-based mutational analysis combined with drug susceptibility assays indicate that drugs are guided through dedicated transport channels toward the drug binding pockets. A co-structure obtained in the combined presence of erythromycin, linezolid, oxacillin and fusidic acid shows binding of fusidic acid deeply inside the T protomer transmembrane domain. Thiol cross-link substrate protection assays indicate that this transmembrane domain-binding site can also accommodate oxacillin or novobiocin but not erythromycin or linezolid. AcrB-mediated drug transport is suggested to be allosterically modulated in presence of multiple drugs.

PubMed: 34188038
DOI: 10.1038/s41467-021-24151-3

実験手法

X-RAY DIFFRACTION (3.05 Å)