6ZNS

Crystal Structure of DUF1998 helicase MrfA

6ZNS の概要

• エントリーDOI: 10.2210/pdb6zns/pdb
• 関連するPDBエントリー: 6ZNP 6ZNQ
• 分子名称: Uncharacterized ATP-dependent helicase YprA, ZINC ION (2 entities in total)
• 機能のキーワード: dna, repair, helicase, mrfa, ypra, duf1998, hydrolase
• 由来する生物種: Bacillus subtilis subsp. subtilis str. 168
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 84899.21

構造登録者

Roske, J.J.,Liu, S.,Loll, B.,Neu, U.,Wahl, M.C. (登録日: 2020-07-06, 公開日: 2020-11-25, 最終更新日: 2024-11-13)

主引用文献

Roske, J.J.,Liu, S.,Loll, B.,Neu, U.,Wahl, M.C.
A skipping rope translocation mechanism in a widespread family of DNA repair helicases.
Nucleic Acids Res., 49:504-518, 2021

PubMed Abstract: Mitomycin repair factor A represents a family of DNA helicases that harbor a domain of unknown function (DUF1998) and support repair of mitomycin C-induced DNA damage by presently unknown molecular mechanisms. We determined crystal structures of Bacillus subtilis Mitomycin repair factor A alone and in complex with an ATP analog and/or DNA and conducted structure-informed functional analyses. Our results reveal a unique set of auxiliary domains appended to a dual-RecA domain core. Upon DNA binding, a Zn2+-binding domain, encompassing the domain of unknown function, acts like a drum that rolls out a canopy of helicase-associated domains, entrapping the substrate and tautening an inter-domain linker across the loading strand. Quantification of DNA binding, stimulated ATPase and helicase activities in the wild type and mutant enzyme variants in conjunction with the mode of coordination of the ATP analog suggest that Mitomycin repair factor A employs similar ATPase-driven conformational changes to translocate on DNA, with the linker ratcheting through the nucleotides like a 'skipping rope'. The electrostatic surface topology outlines a likely path for the displaced DNA strand. Our results reveal unique molecular mechanisms in a widespread family of DNA repair helicases linked to bacterial antibiotics resistance.

PubMed: 33300032
DOI: 10.1093/nar/gkaa1174

実験手法

X-RAY DIFFRACTION (3.32 Å)