6ZHX

Cryo-EM structure of the regulatory linker of ALC1 bound to the nucleosome's acidic patch: nucleosome class.

6ZHX の概要

• エントリーDOI: 10.2210/pdb6zhx/pdb
• EMDBエントリー: 11220
• 分子名称: Histone H3, Histone H4, Histone H2A type 1, ... (8 entities in total)
• 機能のキーワード: alc1, chd1l, chromatin remodeler, dna damage response, nucleosome, nuclear protein, gene regulation, dna binding protein
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 206785.14

構造登録者

Bacic, L.,Gaullier, G.,Croll, T.I.,Deindl, S. (登録日: 2020-06-24, 公開日: 2020-12-23, 最終更新日: 2024-07-10)

主引用文献

Lehmann, L.C.,Bacic, L.,Hewitt, G.,Brackmann, K.,Sabantsev, A.,Gaullier, G.,Pytharopoulou, S.,Degliesposti, G.,Okkenhaug, H.,Tan, S.,Costa, A.,Skehel, J.M.,Boulton, S.J.,Deindl, S.
Mechanistic Insights into Regulation of the ALC1 Remodeler by the Nucleosome Acidic Patch.
Cell Rep, 33:108529-108529, 2020

PubMed Abstract: Upon DNA damage, the ALC1/CHD1L nucleosome remodeling enzyme (remodeler) is activated by binding to poly(ADP-ribose). How activated ALC1 recognizes the nucleosome, as well as how this recognition is coupled to remodeling, is unknown. Here, we show that remodeling by ALC1 requires a wild-type acidic patch on the entry side of the nucleosome. The cryo-electron microscopy structure of a nucleosome-ALC1 linker complex reveals a regulatory linker segment that binds to the acidic patch. Mutations within this interface alter the dynamics of ALC1 recruitment to DNA damage and impede the ATPase and remodeling activities of ALC1. Full activation requires acidic patch-linker segment interactions that tether the remodeler to the nucleosome and couple ATP hydrolysis to nucleosome mobilization. Upon DNA damage, such a requirement may be used to modulate ALC1 activity via changes in the nucleosome acidic patches.

PubMed: 33357431
DOI: 10.1016/j.celrep.2020.108529

実験手法

ELECTRON MICROSCOPY (2.5 Å)