6ZGA

COPII on membranes, inner coat

6ZGA の概要

• エントリーDOI: 10.2210/pdb6zga/pdb
• EMDBエントリー: 11199
• 分子名称: Protein transport protein SEC23, Protein transport protein SEC24, Small COPII coat GTPase SAR1, ... (8 entities in total)
• 機能のキーワード: protein transport, secretion, trafficking
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 422935.92

構造登録者

Zanetti, G.,Hutchings, J.,Cheung, A.C.M. (登録日: 2020-06-18, 公開日: 2021-02-17, 最終更新日: 2025-04-09)

主引用文献

Hutchings, J.,Stancheva, V.G.,Brown, N.R.,Cheung, A.C.M.,Miller, E.A.,Zanetti, G.
Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network.
Nat Commun, 12:2034-2034, 2021

PubMed Abstract: COPII mediates Endoplasmic Reticulum to Golgi trafficking of thousands of cargoes. Five essential proteins assemble into a two-layer architecture, with the inner layer thought to regulate coat assembly and cargo recruitment, and the outer coat forming cages assumed to scaffold membrane curvature. Here we visualise the complete, membrane-assembled COPII coat by cryo-electron tomography and subtomogram averaging, revealing the full network of interactions within and between coat layers. We demonstrate the physiological importance of these interactions using genetic and biochemical approaches. Mutagenesis reveals that the inner coat alone can provide membrane remodelling function, with organisational input from the outer coat. These functional roles for the inner and outer coats significantly move away from the current paradigm, which posits membrane curvature derives primarily from the outer coat. We suggest these interactions collectively contribute to coat organisation and membrane curvature, providing a structural framework to understand regulatory mechanisms of COPII trafficking and secretion.

PubMed: 33795673
DOI: 10.1038/s41467-021-22110-6

実験手法

ELECTRON MICROSCOPY (4.6 Å)