6ZAZ

Fructo-oligosaccharide transporter BT 1762-63

6ZAZ の概要

• エントリーDOI: 10.2210/pdb6zaz/pdb
• 関連するPDBエントリー: 6Z8I 6Z9A
• 分子名称: SusD homolog, SusC homolog, beta-D-fructofuranose-(2-6)-beta-D-fructofuranose-(2-6)-beta-D-fructofuranose-(2-6)-[beta-D-fructofuranose-(2-1)]beta-D-fructofuranose-(2-6)-beta-D-fructofuranose-(2-6)-beta-D-fructofuranose, ... (8 entities in total)
• 機能のキーワード: outer membrane protein tonb-dependent transporter susc susd levan bacteroides thetaiotaomicron, membrane protein
• 由来する生物種: Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 184449.18

構造登録者

van den Berg, B. (登録日: 2020-06-06, 公開日: 2020-11-18, 最終更新日: 2024-10-09)

主引用文献

Gray, D.A.,White, J.B.R.,Oluwole, A.O.,Rath, P.,Glenwright, A.J.,Mazur, A.,Zahn, M.,Basle, A.,Morland, C.,Evans, S.L.,Cartmell, A.,Robinson, C.V.,Hiller, S.,Ranson, N.A.,Bolam, D.N.,van den Berg, B.
Insights into SusCD-mediated glycan import by a prominent gut symbiont.
Nat Commun, 12:44-44, 2021

PubMed Abstract: In Bacteroidetes, one of the dominant phyla of the mammalian gut, active uptake of large nutrients across the outer membrane is mediated by SusCD protein complexes via a "pedal bin" transport mechanism. However, many features of SusCD function in glycan uptake remain unclear, including ligand binding, the role of the SusD lid and the size limit for substrate transport. Here we characterise the β2,6 fructo-oligosaccharide (FOS) importing SusCD from Bacteroides thetaiotaomicron (Bt1762-Bt1763) to shed light on SusCD function. Co-crystal structures reveal residues involved in glycan recognition and suggest that the large binding cavity can accommodate several substrate molecules, each up to ~2.5 kDa in size, a finding supported by native mass spectrometry and isothermal titration calorimetry. Mutational studies in vivo provide functional insights into the key structural features of the SusCD apparatus and cryo-EM of the intact dimeric SusCD complex reveals several distinct states of the transporter, directly visualising the dynamics of the pedal bin transport mechanism.

PubMed: 33398001
DOI: 10.1038/s41467-020-20285-y

実験手法

X-RAY DIFFRACTION (2.69 Å)