6Z99

Copper transporter OprC

Summary for 6Z99

• Entry DOI: 10.2210/pdb6z99/pdb
• Related: 6FOK 6FOM
• Descriptor: Putative copper transport outer membrane porin OprC, SILVER ION (3 entities in total)
• Functional Keywords: copper transporter, tbdt, membrane protein, pseudomonas aeruginosa, oprc
• Biological source: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
• Total number of polymer chains: 2
• Total formula weight: 159373.80

Authors

Bhamidimarri, S.P.,van den Berg, B. (deposition date: 2020-06-03, release date: 2021-06-30, Last modification date: 2024-01-24)

Primary citation

Bhamidimarri, S.P.,Young, T.R.,Shanmugam, M.,Soderholm, S.,Basle, A.,Bumann, D.,van den Berg, B.
Acquisition of ionic copper by the bacterial outer membrane protein OprC through a novel binding site.
Plos Biol., 19:e3001446-e3001446, 2021

PubMed Abstract: Copper, while toxic in excess, is an essential micronutrient in all kingdoms of life due to its essential role in the structure and function of many proteins. Proteins mediating ionic copper import have been characterised in detail for eukaryotes, but much less so for prokaryotes. In particular, it is still unclear whether and how gram-negative bacteria acquire ionic copper. Here, we show that Pseudomonas aeruginosa OprC is an outer membrane, TonB-dependent transporter that is conserved in many Proteobacteria and which mediates acquisition of both reduced and oxidised ionic copper via an unprecedented CxxxM-HxM metal binding site. Crystal structures of wild-type and mutant OprC variants with silver and copper suggest that acquisition of Cu(I) occurs via a surface-exposed "methionine track" leading towards the principal metal binding site. Together with whole-cell copper quantitation and quantitative proteomics in a murine lung infection model, our data identify OprC as an abundant component of bacterial copper biology that may enable copper acquisition under a wide range of conditions.

PubMed: 34762655
DOI: 10.1371/journal.pbio.3001446

Experimental method

X-RAY DIFFRACTION (2.68 Å)