6Z6W

Poliovirus type 3 (strain Saukett) stabilised virus-like particle (PV3 SC8) from a mammalian expression system.

6Z6W の概要

• エントリーDOI: 10.2210/pdb6z6w/pdb
• EMDBエントリー: 11106
• 分子名称: Capsid proteins, VP1, Capsid proteins, VP0, Capsid proteins, VP3, ... (4 entities in total)
• 機能のキーワード: capsid protein, virus like particle
• 由来する生物種: Human poliovirus 3; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 97800.40

構造登録者

Bahar, M.W.,Porta, C.,Fry, E.E.,Stuart, D.I. (登録日: 2020-05-29, 公開日: 2020-12-30, 最終更新日: 2024-07-10)

主引用文献

Bahar, M.W.,Porta, C.,Fox, H.,Macadam, A.J.,Fry, E.E.,Stuart, D.I.
Mammalian expression of virus-like particles as a proof of principle for next generation polio vaccines.
NPJ Vaccines, 6:5-5, 2021

PubMed Abstract: Global vaccination programs using live-attenuated oral and inactivated polio vaccine (OPV and IPV) have almost eradicated poliovirus (PV) but these vaccines or their production pose significant risk in a polio-free world. Recombinant PV virus-like particles (VLPs), lacking the viral genome, represent safe next-generation vaccines, however their production requires optimisation. Here we present an efficient mammalian expression strategy producing good yields of wild-type PV VLPs for all three serotypes and a thermostabilised variant for PV3. Whilst the wild-type VLPs were predominantly in the non-native C-antigenic form, the thermostabilised PV3 VLPs adopted the native D-antigenic conformation eliciting neutralising antibody titres equivalent to the current IPV and were indistinguishable from natural empty particles by cryo-electron microscopy with a similar stabilising lipidic pocket-factor in the VP1 β-barrel. This factor may not be available in alternative expression systems, which may require synthetic pocket-binding factors. VLPs equivalent to these mammalian expressed thermostabilized particles, represent safer non-infectious vaccine candidates for the post-eradication era.

PubMed: 33420068
DOI: 10.1038/s41541-020-00267-3

実験手法

ELECTRON MICROSCOPY (3 Å)